石斛合剂通过调控Sirt3介导的线粒体自噬通路缓解大鼠糖尿病心肌病

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Abstract：ObjectiveToexplorethemechanismof Shihu Mixture （SHM）forimprovingdiabeticcardiomyopathy.Methods Thirty male SDrats wererandomized into3 groups （n=10）for type2diabetes melitus modeling byhigh-fatand -sugar feeding for12weeksandintraperitonealstreptozotocinijection，followedbytreatment withdailygavageofnoralsaline （model group），metforinutioretactfoweeksitoralledatsaseoalotrolgoup.Figbloo glucoseandcardiacweightindeofteatsweremontoredndtheirG，TC，DL-C，HDL-CndDHlevelsere determined; serum and myocardial levels of NP， CRP， NF- α and IL-6 were detected with ELISA. Myocardial pathological changes and ultrastructures of myocardial mitochondria and autophagosomes were examined with HEand Masson staining andtransoirt proteinsweredetectedwithRT-qPCR，Westernblotingandimmunohistochemistry.ResultsComparedwiththoseinthe control group，theratsintheother3groupsshowedsignificantlyincreasedfastingbloodglucose，cardiacweightindexsum TC， TG，LDL-C，LDH， CRP andBNPlevelsand myocardial levelsof TNF ⋅α and IL-6 with lowered HDL-Clevel， obvious myocardialadoorialtholgiesddglatedexpesofirt3，oxOap-oaK1arind P62.TreatmentoftheratmodelswithSHMextractsignificantlyreducedfastingblood glucoselevelandcardiacweightindex， loweredeelsoH，P，P ⋅α， IL-6，TC，G，ad-C，ireasedH-Cel，alleviatedmyocardiald mitochondrial damages，promoted autophagosome formation，and improved dysregulation of mitochondrial autophagyrelated geneexpression，showingsimilarefects tometformin.ConclusionSHMalleviatesmyocardialdamageandimproves mitochondrialfunctioninrats withdiabeticcardiomyopathybyregulating themitochondrialautophagypathway throughSirt3. Keywords： diabetic cardiomyopathy; Shihu Mixture; mitophagy; Sirt3 pathway

糖尿病及其并发症糖尿病心肌病（DCM已成为全球流行病，目前全球约有4.63亿人受其影响，预计到2045年将增至7亿例。（剩余16263字）