颈椎病颈椎终板软骨的转录组学特征及针刀干预对FGF18/Akt 轴关键分子表达的影响

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Abstract：ObjectiveToexplorethetranscriptomiccharacteristicsoftheendplatecartilageoftheervicalintervertebaldiscn cervicalspondylosis （CS）andtheefects ofacupotomyonexpressionsoffibroblast growthfactor18（FGF18）/proteinkinase B （Akt）axiskeymolecules.MethodsTranscriptomicanalyses wereperformedusingbioinformaticsmethodsbasedontheGEO database.Inalepimnt，24eaandbitsredodalltotol，oelto groups.Inthelatter twogroups， CSmodels wereestablished followed7dayslaterbyacupotomyintervention （nceaweek for 3weeks）ornoparticulartreatment.Histopathologicalchangesandcellapoptosisintheintervertebraldiscswereexamined withHEstainngandUNELassayndthemRNAexpressonsofGF18，GFR3，andAktintheintervertebraliscswere detected using RT-qPCR;the localizationand expressions ofFGF18，p-Akt，and Akt proteins were examined with immunohistochemistry.ResultsTheendplatecartilageinCSexhibitednumerousdiferentiallyexpressedgenesenrichedin thePI3K-Aktsignaling，calciumsignalingandRap1signalingpathways.CSabbitsshowedobviouschangesinthecevical spinecurvatureandjointdegenerations，changesincervicalintervertebraldisctexture，thinningoftheannulusfibrosus， shrinkageorevenabsenceofthenucleuspulposus，andincreasedapoptoticcelsintheendplatecartilage，whichwereall obviouslyalleviatedafteracupotomy.Nosignificantdiferences werefoundinthemRNAexpressionsofFGF18，FGFR3and Aktin thecervical intervertebral discs among the3 groups.Acupotomy significantlyincreased FGF18 and p-Akt protein expresionsandreducedtheAkt/p-AktratiointhecervicalendplatecartilageofCSrabbits.ConclusionThecervicalendplate cartilageofCSshownumerousdiferentiallyexpressed genes.Acupotomymaydelaydegenerativechangesof the intervertebral discsandimproves CSbyactivatingtheFGF18/Aktaxistoreduceapoptosisofendplatecartilagecells.

Keywords：cevicalspondylosis;acupotomy;transcriptomecharacteristics;intervertebraldisc;FGF18/Aktais

颈椎病（CS）是指颈椎椎间盘退行性改变及其继发病理改变累及周围组织结构从而出现一系列临床表现的疾病，属中医“项痹"“项强"等范畴，欧美地区常称之为颈痛[1-3]。（剩余13391字）