基于GEO筛选的心肌梗死特征性IncRNA分子标志物及实验验证研究

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Identification of Characteristic IncRNA Molecular Markers for Acute Myocardial Infarction Based on GEO Database Screening and Experimental Validation

SHAN Yuanqi1，CHEN Rui 2 ，CHEN Xianru³，DU Wentao³

（1. Hebei Universityof Enginering，Handan O5603，Hebei，China；2.Department of Cardiology，HandanCommercial WorkersHospital，HandanO56O1，HebeiChina；3.DepartmentofCardiology，AfliatedHospitalofHebeiUnivesityof Engineering，Handan O56004，Hebei，China）

【Abstract】ObjectiveToinvestigatetheregulatoryoleofthelongnoncodingRNAANRILinmycardialischemia-reperfusioninjury （MIRI）asociatedwithacutemyocardialinfarction（AMI）andtoidentifypotentialtherapeutictargetsforMIRIintervention. MethodsDiferentillyexpressedgeneswereidentifiedfromanAMdataset（GSE660）obtainedfromGEOdatabase.Keycandidate genes werescreenedusingtreemachne-leaingalgorits：andomforest，lastabolutesrikageandselectionoperato，ndsupportctor machine-recursivefeatureelimination.ThbiologicalfunctionofANRILwasfurthervalidatedinahypoxia/reoxygenationmodelusingthe mouseatrialdellifKee assys，adWestenblotiganalysisesultsAtotalof7relatddierentiallepressdgneseredentifd.Kpahy enrichment analysis revealed significant enrichment in inflammation-and apoptosis-related pathways（ P<0.05） . Five key genes were selected basedonetesftsdskioosbslstucd，cod asignificantassciationwithAM.CellexpermentsdmostratedtatAIexpresionassignificantlyupregulatedinoia reoxygenation model（ P <0.05）. Interference with ANRIL expression markedly reduced the apoptotic rate（ P<0.05 ），increased cell activity （ P<0.05 ），enhanced SOD activity，and decreased MDAlevels（ P<0.05 ），while regulating the expression levels of apoptosis related proteins such asB-cell lymphoma-2，B-cell lymphoma-2-associated X protein，and caspase- 3（P<0.05）. ConclusionlncRNA ANRIL is upregulated inAMI-aociatedMandayexacerbatemyoadialijurybymodlatigapoptosisandoidativesspathways，dhsseavaue as a potential intervention target.

【Keywords】Acutemyocardialinfarction；Myocardialischemia-reperfusionijury；Dierentiallyexpressdgene；ANI;Long noncoding RNA

急性心肌梗死（acutemyocardialinfarction，AMI）是在冠状动脉粥样硬化基础上形成急性血栓导致的严重心血管事件，具有高发病率和高致死率特点[1]尽管再灌注治疗和急救体系不断进步，患者远期仍面临心力衰竭、心律失常等并发症风险[2]。（剩余11413字）