沉默信息调节因子2通过抑制铜死亡改善缺氧/复氧诱导的新生大鼠心肌细胞损伤

打开文本图片集

SIRT2 Alleviates Hypoxia/Reoxygenation-Induced Injury in Neonatal Rat Cardiomyocyte by Inhibiting Cuproptosis

HUANG Dan，ZHAO Shuhong，WANG Shiying，MA Zhenguo

【Abstract】ObjectiveToinvestigatetheefectsilentinformationregulator2（IRT）onhypoxia/reoxygenation（H/R）-induced cardiacnjuryitsunderlyingmechanism.Methdseoatalratardiomcyte（NCM）wastransctedwithspificsallintefering RNA（siRNA）toknockdownSexpresionsubjectedto/Rorposptebuffredsaline（BS）control.Tecellsweredividedinto four groups：siRNA-NC group，si-SIRT2 group，siRNA-NC + H/R group， si-SIRT2 + H/R group. Successful SIRT2 knockdown was confirmed byWestemblotigodalijuysedyeasuigellbilitycatedgas（D）ndaia（CKMB）levels.TecoeriocontentinNCwasevauatedusingcopperionfuorescentprobediydrolipoamideS-acetyltraserase （DLAT）oligomerzationasdetectedbyimunoluorescce.TeexpresionlevelstagetmoleculeseredeternedbyWstebloing quantitativereal-time PCR.ResultsTransfection with si-SIRT2 reducedSIRT2 protein expresion byapproximately 82% （ P<0. 05 ）. H/R markedlysuppressedcardiomyocteviabilitysignifcantlyelevated LDHCK-MBelease.SIT2 knockdownfurther exacbated H/R-inducediuhastally，iseditacellaoperuondlgezatioreado（FDX-1）expressionndupregatedheatsockprotein7（HSP7）.Thsecuprotosis-elatederaiosereitesifedy deficiency.ConclusionSIRT2isindispensableforprotectingNRCMagainstH/Rijury.Itsknockdowndisruptscopperhomeostasis， promotesDLAgmzationdalesDX-1HSP7xpresio，trebygavtigcaoyocyedamage.Thsefingt that SIRT2 may serve as a potential therapeutic target for myocardial protection.

【words】 Silent information regulator 2;Hypoxia/reoxygenation;Cuproptosis

缺血性心脏病（ischemic heartdisease，IHD）是全球主要心血管疾病负担。（剩余9941字）