CD226在子痫前期胎盘中的表达及其对滋养细胞功能的研究

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【中图分类号】 R714.24+5 【文献标识码】A

【Abstract】 Objective To analyze the expresson characteristics of CD226 in placental tissues from preeclampsia （PE） patients and its biological function in trophoblast cels. Methods CD226 mRNA transcript levels and protein expression in placental tissues from the PE patients and women with normal pregnancies were detected by qRT-PCR and immunohistochemical staining. In the trophoblast cell lines HTR8/SVneo and JAR，CD226 gene was knocked down or overexpressed， respectively， to evaluate the impact of its expression changes on cell proliferation， apoptosis， migration，and invasion.Western blot analysis was used to detect the effects of CD226 on the protein expression of TNF-α and NF- σκB p65. Results Compared with the normal pregnancy group， the expression level of CD226 was significantly up-regulated in the placental tissues of the PE group. Knockdown of CD226 enhanced the proliferation，migration，and invasion，andsuppressed apoptosis in HTR8/Svneo cels. Conversely，the overexpression of CD226 significantly inhibited the proliferation， migration，and invasion of JAR cells， while promoting apoptosis in these cels. Furthermore， Western blot analysis revealed that CD226 overexpression promoted the protein expression of TNF- α and NF- κB p65 in trophoblast cells.Conclusion The expression level of CD226 in placental tissues of PE is higher than that in normal pregnancy group. CD226 exerts the inhibitory efects on the proliferation，migration，and invasion，and promotes apoptosis in trophoblast cells.

【Keywords】Preeclampsia; CD226; Placental tissue; Proliferation; Invasion

子痫前期（preeclampsia，PE）是妊娠期高血压疾病常见亚型之一，发病率约为2%～8%[1-2]。（剩余14511字）