红杉醇调节Hippo信号通路对H₂O₂诱导的心肌细胞损伤的影响

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关键词心肌细胞损伤；红杉醇；Hippo信号通路； H2O2 ；实验研究 doi：10.12102/j.issn.1672-1349.2025.21.006

Abstract Objective：To explore the effect of Sequoiol on induced myocardial cell injury by regulating the Hippo signaling pathway. Methods：Human myocardial cels AC16 were divided into the control group， H2O2 group，low，medium，and high-dose Sequoiol groups （L-SequoiolgropequoigroupHSequgroup）ndU-goupEdUsainigsaplidtetectC1celifatio Theapoptosis of AC16 celswasdetectedbythe Hoechst 33258 staining method. γ -H2AX immunofluorescence assaywasapplied to detecthelevelofDNAdamageinAC16cels.Theenzye-linkedimmunosorbentassay（ELISA）methodwasusedtodetectthelevelsof superoxide dismutase（SOD）andreactiveoxygen species（ROS）in theserumof AC16cels.Theproteinimmunobloting method（Westem Blot） was used to detect the protein expressions of YAP1，p-YAP1，TAZ， γ+12AX ，B-cell lymphoma 2（Bcl-2），Bcl-related X protein（Bax）， andCaspase-3 in AC16 cels.Results：Compared with the control group，the EdU positive cellrate，SOD，and p -YAP1，Bcl-2protein expression of AC16 cells in the H2O2 groupdecreased，the apoptosisrate，DNA damage，ROS，YAP1，TAZand ，Bax，and Caspase-3proteinexpression levels increased（ P<0.05 ）;comparedwiththe H2O2 group，the EdU positive cell rate，p-YAP1，SOD，and Bcl-2 proteinexpressonincreasedintheLequoilgroup，SequilgroupandHSeqoiolgroup，teapoptosisrate，damage and YAP1，TAZ， γ -H2AX，Baxaspase-3proteixpreinvelsreaedadastecetratioofoolieadih gradually increasing ordecreasing trend（ P<0.05 ;the EdU positive cell rate，SOD and p-YAP1，Bcl-2 in the XMU-MP-1 group were lower than those in the H-Sequoiol group，the apoptosis rate，DNA damage，ROS and YAP1，TAZ， γ+H2Ax ，Bax，Caspase-3protein expression inthe H-Sequoiol group were higher than those in the H-Sequoiol group（ P<0.05 ）.Conclusion： Sequoiol can inhibit H2O2 induced myocardial cellinjury，and its mechanism relates regulating the Hippo signaling pathway.

Keywordsmyocardial cell injury;Sequoiol; Hippo signaling pathway; H2O2; experimental study

随着人口老龄化的加速和不良生活方式的影响，心血管疾病的危险因素不断增加，心肌梗死的发病率和死亡率呈上升趋势[1]。（剩余9152字）