明君散对动脉粥样硬化大鼠TLR4/MyD88/NF-κB信号通路的影响

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doi：10.12102/j.issn.1672-1349.2025.14.008

TheEffectofMingjun Powderonthe TLR4/MyD88/NF- κB Signaling Pathway in Atherosclerotic Rats CHESihua1，YANG Na2 ZHENGWenyu²，ZHANGShumao？，SHI Guijun²

1.ChangchunUversityfineedicinanghn30oinina;angnspitalofaditioalCie Changchun3ooo，Jilin，China;3.JlinProvincialDisabledPersons FederationRehabilitationHospital

CorrespondingAuthor SHlGuijun，E-mail：zsmsgj @ 163.com

AbstractObjective：ToxploretheefectofMingjunPowderonatheroscleroticratsbasedonteTollikereceptor4（R4）eloid differentiation protein 88（MyD88）/nuclear factor- κB（NF-κB） signaling pathway.Methods：The atherosclerotic model of rats was established byhigh-fat feed combinedwith vitamin method.The rats were divided into the blank group，the model group，the Western medicine group[atorvastatin 1mg/（kg⋅d）] ，the low-dose Mingjun Powder group[2.35 mg/（kg⋅d）] ，the medium-dose Mingjun Powder group[4. 70mg/（kg⋅d）] and the high-dose Mingjun Powder group [9.40mg/（kg⋅d）] ，with 10 rats in each group.After gavaging for eight weeks，the levels of serum totalcolestes）otit detected.Hematoxylineosin（HE）stainingwasusedtoobservethehistologicalmorphologicalchangsofthethoracicaortainratsTe expressions of TLR4，MyD88 and NF- κB mRNA in the aorta of rats were detected by reverse transcription polymerase chain reaction （RT-PCR）.The expressionsof TLR4，MyD88，and NF-κB proteinsintheaorta of ratsweredetected byWestern Blot.Results：Compared withthe modelgroup，thelevelsofserumTC，TGandLDL-Cin theWestemmedicinegroup，themedium-dseMingjunPowdergroup， and the high-dose Mingjun Powder group decreased，and the level of HDL-C increased P<0.05 or P<0.01 ）.The expressions of TLR4， MyD88， NF- κB mRNA and protein in the aortic tisues ofrats in the Westem medicine groupand each dose Mingjun Powder groupsall decreased（ P<0.05 or P<0.01 ）.Conclusion：Mingjun Powder might playananti-atherosclerotic efect by inhibiting the TLR4/MyD88/NF-kB signaling pathway.

Keywordsatherosclerosis；MingjunPowder;Tollikereceptor4/myeloid diferentiationprotein88/nuclearfactor- κB signaling pathway; blood lipid; rats; experimental study

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