三七总皂苷对大鼠心肌缺血再灌注损伤的影响及抗焦亡机制研究

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doi：10.12102/j.issn.1672-1349.2025.14.007

EfectsofPanaxNotoginsengSaponinsonMyocardiallschemia-reperfusionInjuryinRatsandtheMechanismofAnti-pyroptosis ZHAOWeikun1，UTongtong'，UXiangwe，QNYang1，LUFengxia1，Yuting²，HUANGYul，OQ，UHuaxin，Ue

1.TheFirst AfaesiafuindaUveitunag，ina;uiicavsit99 Guangxi， China

Corresponding AuthorQINYang，E-mail：1258778506@qq.com;LYU Xiangwei，E-mail：lvxiangwei910 @ 163.com

AbstractObjective：ToobservetheefectofPanaxnotoginsengsaponins（PNS）onmyocardialischemia-repefusion（I）injuryand exploreitsmechanismrelatedtotheregulationofcardiomyocytepyroptosis.Methods：EighteenadultmaleSDratsweredividedintosham group，IR groupandPSgroupbyrandomnumbertablemethod，with6ratsineach group.Themodelwaspreparedbyligatigtheleft anteriordescendingcoronaryarteryAD）ofratswithischemiafor30minutesandreperfusionfor2hours.Theatsinthesamgroupand the IR groupwere intraperitoneally injectedwith normal saline（ 10mL/kg） ，while those in the PNS groupwere intraperitoneally injected with （20 PNS（50mg/kg） 30 minutes before modeling.The degree of myocardial injury was observed by hematoxylin-eosin（HE） staining.The expresionofcsteineaspartatespecicprotease-1Caspase1）asdetectedyimmunoistochemistryeexpresiosofCaspse1 andgasdermin D（GSDMD）proteincontentsinmyocardialtisseweredetectedbyWesternBlotResuts：Comparedwiththeshamgroup， thedegree ofmyocardialinjuryintheIRgroupwasaggravated，andtheproteinexpressionsofaspase-1andGDinceased P< 0.05）.ComparedwiththeIRgroup，thedegreeofmyocardialinjuryinthePNSgroupwasaleviatedandtheproteinexpressionsof Caspase-1 and GSDMD decreased（ ⋅∠1.5′ .Conclusion：Panax notoginseng saponins could alleviate myocardial iR injury in rats，and its mechanism might relate to the inhibition of cardiomyocyte pyroptosis.

Keywordsmyocardialiscemiareperfusioninjuypanaxotogisengsapons;pyptosis;csteineaspasticaidspeificote1 （Caspase-1） ; gasdermin D（GSDMD）; rats; experimental study

急性心肌梗死（acutemyocardial infarction，AMI）发生时，早期治疗可恢复缺血心肌的血液供应，降低死亡风险；持续的缺血状态导致心肌细胞出现不可逆性损害。（剩余9283字）