基于HPLC指纹图谱和有效成分含量评价不同产地草豆蔻质量

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中图分类号R284；R917 文献标志码A 文章编号 1001-0408（2025）18-2262-05

DOI 10.6039/j.issn.1001-0408.2025.18.08

Evaluationof thequalityofAlpiniakatsumadaifromdifferent habitatsbasedonHPLCfingerprintand determinationofactiveingredientcontent

LIAO Yanwu1，WANG Yingjun’，LIU Shun23，QIU Mingming1， ZHU Yufeng1 （1. Dept. of Pharmacy，the Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine， Nanjing 210029，China; 2.Jiangsu Engineering Research Centre for Innovative Chinese Medicine Preparations， Nanjing 210029，China；3.The First Clinical Medical College，Nanjing University of Chinese Medicine， Nanjing 210023，China）

ABSTRACTOBJECTIVEToevaluatethequalitydiferencesofAlpinia katsumadaifrom differenthabitats.METHODSHighperformance liquidchromatography（HPLC）wasusedtoestablishthefingerprintsofA.katsumadai from18batchesofdiferent habitats，andthequaityofA.katsumadaifromdiferenthabitatswascomprehensivelyevaluatedbysimilarityevaluation，cluster analysis（CA），principalcomponentanalysis（PCA），orthogonalpartialleastsquares-discriminantanalysis（OPLS-DA）andthe content determinationresultsof alpinetin，pinocembrin，cardamoninandalnustoneinA.katsumadai.RESULTS Thesimilarityof HPLC fingerprints for 18 batches of A. katsumadai was ${ ＼tt > } 0 . 9 ＼$ .Eleven common peaks were identified from the chromatogram，and fourof thm werespecificallycharacterized.Both CAandPCA grouped18batchesofA. katsumdainto3categories，extracting2 principal components （the cumulative variance contribution rate reached 89.798% ）.OPLS-DA identified 9 quality difference markers，namelythecomponentscorrespondingtopeaks4，9，3，2，7（pinocembrin），8（cardamonn），6（alpinetin），and11 （alnustone）.Thecontentofalpinetin，piocembrin，cardamonin，andalnustonerangedfrom4.5071-11.5797，5.1544-14.183， 5.109 5-13.588 3 and 4.494 6-11.277 2mg/g ，respectively. CONCLUSIONS The quality of A. katsumadai from different habitats is quite different，and the quality of A katsumadai from Hainanis the best.

KEYWORDSAlpiniakatsumadai；HPLCfgerprint；chemicalpaterecogtion；ontentdetenation；habitats；qualitycotrol

草豆蔻始载于《名医别录》，为姜科植物草豆蔻AlpiniakatsumadaiHayata的干燥近成熟种子，具有燥湿行气、温中止呕的功效；临床上主要用于寒湿内阻、皖腹胀满冷痛、暖气呕逆、不思饮食等证的治疗[3。（剩余9029字）