HPLC法测定金匮肾气丸中茯苓酸的含量

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【中图分类号】R284.1 【文献标志码】A 【文章编号】1007-8517（2025）10-0053-04

DOI： 10.3969/j . issn.1007 -8517. 2025. 10. zgmzmjyyzz202510010

DeterminationofPachymicAcidinJinguiShenqiPillbyHPLC

CAO Puqiong1YU Jikui²MA Guangyu³*LI Dongliang³ 1.Baoshan Inspection and Testing Center for Food and Drug，Baoshan 678OOo，China; 2. Baoshan Maternal and Child Health Hospital，Baoshan 678OOo，China; 3.BaoshanCollege of Traditional Chinese Medicine，Baoshan678OOO，China

Abstract：Objective Toestablishamethod forthedeterminationof pachymicacid inJingui Shenqi Pill.Methods High perforance liquid chromatography（HPLC）was used to determinethe content of pachymic acid.Shiseido CAPCELL PAK C18 （ 4.6mm× 250mm ， 5μm ）was used as the chromatographic column，and the mobile phase was acetonitrile -0.1% phosphoric acid aqueous solution（75：25）.The detection wavelength was 2O8 nm，injection volume 20μL ，column temperature 30‰ .Results The linear relationship of pachymic acid was good in the range of 2.92-58.40μg/mL （ r=0.9995 ）. The average recovery was 95.65% （RSD =1.02% ）.Conclusion The established method issimple，specific and reproducible，and can be used for the determinationof pachymic acid in Jingui Shenqi Pill.

Key words：Jingui Shenqi Pill；Pachymic Acid；High Performance Liquid Chromatography；Content Determination

金匮肾气丸为中医临床治疗肾气虚常用药，由地黄、山药、酒萸肉、茯苓等10味药材组方，具有温补肾阳、化气行水之功，用于肾虚水肿、腰膝酸软、小便不利、畏寒肢冷等症[1-2]。（剩余4589字）