本氏烟tsRNAs对马铃薯Y病毒侵染的响应

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中图分类号：S435.72 文献标识码：A文章编号：1007-5119（2025）03-0070-11

Altered Expression of Transfer-RNA-derived small RNAs after Potato Virus Y Infection in Nicotiana benthamiana

GAO Xinwen， SONG Hongping， WANG Hui， MIAO Pu3 ， JIAO Yubing1， SHEN Lili'， WANG Yujie3*， YANG Jinguang1 （1.Institute ofTobaccoResearchofChinese Academyof Agricultural Sciences，Qingdao 266101，China; 2.YangtzeUniversity Jingzhou434025，Hubei，China; 3.Luoyang CityCompanyofHenan TobaccoCompany，Luoyang 471oo，HenanChina）

Abstract： To explore the molecular mechanismsunderlyingNicotiana benthamiana responses topotato virus Y（PVY）infection， smallRNA sequencing （sRNA-seq）andtranscriptomesequencing （RNA-seq）were employed toanalyze thediferences intsRNAs andtranscriptionalesponsesinN.benthmianafollowingPVYinfection.Teresultsshowedthat39iferentillyexpressdtNAs （tRNAhalves）and119tRFs（tRNA-relatedfragments）wereidentifiedatthe7thdaypost-inoculation.Enrichmentanalysisof diferentiallexpresedtargetgenes （DETGs）demonstratedtat117and60target genesoftFsinleavesandstems，espectively were predominantlyenrichedinpathwayssuchassignaltransductionandenergymetabolism.Notably，theexpresionoft-1：18- His-GTG-1-M3was mostsignificantlydown-regulated iPVY-infectdleaves.SuppressgtheexpresionoftDR-1：18-Hs-G-1- M3 in N. benthamiana markedly increasingthe expresion levels of its target genes，yabDand RNF170.These findings provide a reference forfurtherin-depthresearchontheroleoftsRNAsinPVY-host plant interactionsandthedevelopmentofPVYcontrol strategies.

Keywords： Nicotiana benthamiana; potato virus Y; small RNA sequencing; transcriptome sequencing

马铃薯Y病毒（potatovirusY，PVY）作为一种单链RNA病毒，具有分布广[]、进化快、种群规模庞大以及高度宿主依赖性等特点，在生产上尚无靶向药剂。（剩余16048字）