烟属40份种质资源的花粉形态及其分类学意义

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中图分类号：S572.02 文献标识码：A文章编号：1007-5119（2025）03-0012-08

Pollen Morphology of 40 Germplasm Resources of Nicotiana and Its Taxonomic Significance

GUO Kaiyang1， WANG Rengang2*， WANG Zili²，LIN Shifeng²， YANG Zhixiao²， ZHANG Jie²，LIU Guoqin1* （1.Collge ofobaccoScience，GuzhouUniversityGuiang55025，China;2.GuizouAcademyofTobacoScience/Molecular GeneticsKeyLaboratory ofChina Tobacco， Guiyang55oo81， China）

Abstract： Toexplorethe palynologicalcharacteristicsof Ncotiana andits taxonomic significane，we examiedpollnmorphology of36wild species and4cultivated varieties using scaning electron microscopyand investigated their taxonomicrelationships throughclusteraalysis.Teresultsevealedthatall4accessionsexibited monad，isopolarplengrainswithtricolpateor tetracolporate apertures， displaying spherical to prolate shapes [P/E=1.66（0.99-2.16）] and measuring 39.96 （26.55-58.08） μm×24.28 （17.83–33.79） μm Seven exine ormamentation types were identified， including verrucate， verrucate-rugulate，rugulate， rugulatestriate，strate，triateeticulate，ndeticlate，amongichtestratetype asthemostprevaent，andmostspecisebitedeine perforations.PolensizeandexineomamentationwereidentifiedasthekeydiagnosticcharacteristicsforNicotianapollen diferentiation.Clusteranalysisbasedoncharacteristic values clasifiedthe40taxaintothreegroupsatasimilaritycoeffcientof 15.73.Thecusteringresultsofixsctions （icotianst.Nicotiaa，icotiansct.Paniculatae，NicotianasctPetides， Nicotian sect.Repandae，Nicotianasect.Tomentosae，andNicotianasect.Trigonophylae）wereconsistent withtheKnapp taxonomicsystem.Thepalyologicalresultsprovidesignificantcoroborativeevidenceforthe taxonomic studyofNicotian，with the classification results generally supporting findings from molecular systematic research.

eywords： Nicotiana; wild species; pollen morphology; electron microscopy scanning; taxonom

烟属（Nicotiana）是茄科（Solanaceae）第五大属，属内成员众多，目前公认的有82个种[1]。（剩余15463字）