基于ISSR分子标记的钻天柳遗传多样性分析
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中图分类号：S791.43 文献标识码：A 文章编号：1001-9499（2025）04-0033-07
Analysisof GeneticDiversityinSalixarbutifolia Using ISSR Molecular Markers
ZHANGYu1.2 GAO Jinhui1 XUE Yonghui² JIANG Zhen4 ZHANGGuocai³ ZHANGHeyang ZHANG Houliangl**
（1.Yichun Branch of Heilongjiang Academy ofForestry，Heilongjiang Yichun 153000; 2.Heilongjiang Agricultural Engineearing Vocational College，Heilongjiang Harbin 150088;3.Northeast Forestry University，Heilongjiang Harbin 15o04O;4.Yichun Geographic Information Processing Center，Heilongjiang Yichun 153000）
AbstractThis study established18 natural distribution sites and plots in the Greaterand Lesser Khingan Mountains from 2021 to 2022，collcting 50 samples of Salix arbutifolia.The modified CTAB method was employed for genomic DNA extraction from leaves.Through ISSR-PCR amplification，10 primers were identified，and their optimal annealing temperatures were determined.The results indicate that the extracted genomic DNA meets quality standards.The genetic diversityof Salixarbutifolia populations is relatively high，with an average polymorphic band percentage of 91.91% at the species level and 92.22% overall， indicating substantial genetic variation.The Shannon information index and Nei'sgene diversity index peaked at an elevation of 548m .The genetic differentiation coefficient （GST） was calculated at 0.2303， suggesting significant gene flow among populations.The five natural populations exhibited close genetic relationships，with genetic similarity ranging from O.8867 to 0.9651 and genetic distance from 0.0355 to 0.1203.The 29 individuals were categorized into four major groups.The findings conclude thatthe genetic diversity of Salixarbutifoliaarises from genetic variation influenced by itsbreeding system and seed dispersal mechanisms，while the impact of geographic isolation appears minimal.
Key wordsSalix arbutifolia; ISSR;genetic diversity; genetic composition
钻天柳（Salixarbutifolia）是杨柳科中分布范围最小的一个类群，该树种生长迅速，树形优美，适合作为绿化树种，具有显著的生态价值，常分布于河岸及溪流两侧，是理想的护岸树种，零星分布于中国东北部分地区[1]。（剩余9009字）
