三角槭一步成苗及生根机制
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中图分类号：S792.35 文献标识码：A 文章编号： 1000-4440（2025）07-1422-07
The one-step seedling formation and rooting mechanism of Acer buergerianum Miq.
YAN Kunyuan，LI Shushun， MA Qiuyue， DU Yiming， LI Qianzhong， ZHU Lu（Institute ofLeisure Agriculture，Jiangsu Academy of Agricultural Sciences，Nanjing 21oo14，China）
Abstract：TheAcer buergerianum，renowned for itsornamental foliageand extensive applications，possesses significantaestheticandeconomic value.To facilitatetherapidproductionofhigh-qualityseedlingsandachieveindustrial-scale propagation，this study employed stem segments with budsas explants to screenforoptimal colection times，sterilization methods，and medium formulations foraxillrybud induction androtinduction.A comprehensive isue culture rapid propagationsystemforAcerbuergerianum was established.Further investigationintotherotformation mechanisminvolvedcytologicalanalysisofadventitiousrootprimordiaandtheexpresionlevelsofkeygenesduringrootdevelopment.Thefindings indicated thata combinationof 75% ethanol disinfection for 2O seconds followed by 0.1% mercuric chloride disinfection for 15 minutes was the most efectivesterilization methodfor explants collected in June.Theoptimal medium foraxillry bud induction was MS+0.5mg/L indole-3-butyric acid（IBA），achieving an induction rate of 82.3% .For adventitious root induction，theoptimal medium was 1/2 MS + 0.6 mg/L IBA，resulting in a rooting rate of 88.7% with an average of 4.9rootsperplant.The initiation and growth of adventitiousroot primordiain Acerbuergerianum werecloselyassociatedwith auxinIBA.At aconcentrationof 0.6mg/L IBA，the formation of root primordia was significantly enhanced，and the relative expression levels of key genes Ab PIN1，AbPIN2，and AbPIN3 during root development increasedmarkedly.Thisstudyprovidesatechnicalreferenceforlarge-scalepropagationofnewvarietiesofAcerbuergerianum and establishes afoundation for exploring its root formation mechanism.
Key words： Acer buergerianum Miq.；tissue culture；rapid propagation；rooting
三角槭（AcerbuergerianumMiq.）为无患子科槭属落叶乔木，高度可达 10m ，树冠卵形，原产于中国东部[1-2]。（剩余10170字）
