重楼全长转录组测序及生物信息学分析

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Full-length transcriptome sequencing and bioinformatics analysis of Paris

XU Ping'，ZHANG Xuan’，YU Mengwen¹，XU Honggao1，TU Zhenhua’，ZHANG Xiaoye'

ZHANG Jie'， OU Zhenggang²， CHEN Jia³， ZHENG Guowei1

Abstract：The purpose of this study is to lay a foundation for the systematic exploration of saponin synthesis genesin Paris byobtaining the full-length transcriptome data of Paris. The third-generation sequencing technologyon PacBio Sequel platform was used.In this study，the rhizome mixed samples of Paris polyphylla Smithvar.yunnanensis（Franch.）Hand.-Mazz.，Paris fargesiiFranch.andParisforrestii（Takht.）H.Liwith significantdifferences in saponin content were deeply sequenced，and then the obtained data were comprehensivelyanalyzedbybioinformatics.A total of42771 transcriptswereobtained，of which40945（ 95.73% ）were successfully matched to seven functional databases，namely KEGG，NR，Swiss-Prot，TrEMBL，KOG，GOand Pfam.Through enrichmentanalysisofKEGG pathway，48genesrelatedtocholesterol synthesis were successullyidentified，andtwokindsofkey enzyme genes involved insaponin modification were specifically screened：12O cytochrome P45O enzyme genes and 53 UDP-glucosyltransferasegenes.Additionaly，2327transcriptionfactorsweredetected，belonging to71transcriptionfactor families.Atotalof27451microsatelitesequences（SSR）werefoundbyMISAanalysis，therewerethemostcoincident types with overlapping SSR，accounting for 32.88% of all repeat types.With the help of high-throughput full-length transcriptome sequencing technology，thetranscriptomeof rhizome tisueof Pariswassystematicallyanalyzed，and thecandidategens andregulatoryelements closelyrelated tosaponinsynthesis weresuccessfully excavated.Thesefindings laythe groundwork for further study onthe molecular mechanism of saponin biosynthesis in Paris.

Key words： Paris；full-length transcriptome；function annotation；saponin

重楼属（ParisL.）植物为藜芦科（Melanthiace-ae）多年生草本植物。（剩余14706字）