沉默信息调节因子1调控成骨成血管功能促进颌骨缺损愈合的实验研究

打开文本图片集

[中图分类号]R782 [文献标志码] A[doi] 10.7518/gjkq.2025046

Promotionof mandibulardefect healing through the regulation of osteogenic and angiogenic functions bysirtuin1

LiuZhikai，LiuHanghang2，LiuShibo，LiBolun，LiuYao，Luo

1.StateKeyLaboratoryofOralDiseases&National CenterforStomatology&National ClinicalResearchCenter for OralDiseases&Dept.ofOrthognathicandJointSurgery，WestChina HospitalofStomatologySichuan University Chengdu61o041，China；2.StateKeyLaboratoryofOral Diseases&National CenterforStomatology&National Clinical Research Center forOral Diseases&Dept.ofEmergency，West China HospitalofStomatologySichuan University Chengdu 610041，China

Supportedby：NationalNaturalScienceFoundationofChina（8237o932）；KeyResearchandDevelopmentProjectofSi chuanProvince （2024YFFK0204）；Natural ScienceFoundation Youth ProjectofSichuan Province（2023NSFSC1512） Correspondence：Luo En，Email： luoen52l125@sina.com

[Abstract]ObjectiveThis studyaims to investigate the effectofsirtuin1（SIRT1）onosteogenic and angiogenic functions inmiceunder in vivoand in vitroconditions，aswellasitseffectonmandibulardefect healing.MethodsSIRT1activatorsand inhibitors wereused to intervene in MC3T3-E1cellsand mandibulardefects inmice.Various methods，includingcellcountingkit-8（CCK-8）assyreal-timequantitativepolymerasechainreaction，Westernblot，alkalinephos

phatase staining，and immunofluorescence staining，were employedtostudythe influenceofSIRT1 ontheexpression of osteogenic and angiogenic factors in MC3T3-E1 cells，aswell as the healing and osteogenic and angiogenic functions of mandibular defects in mice.Results Ininvitro experiments， the activation of SIRT1 promoted the expression of osteogenic and angiogenic factors

inMC3T3-E1cels.Ininvivoexperiments，SIRT1activationfacilitatedthehea-lingofmandibulardefectsandenhanced theosteogenicandangiogenic functionsofthe mandibular defects.Conversely，the inhibitionofSIRTl activitysuppressed the aforementioned processes.ConclusionSIRT1 can promote the healing of mandibular defects by regulating the osteogenic and angiogenic functions in mice.

[Key words] sirtuinl；angiogenesis；mandibular defect； bone regeneratior

颌骨缺损可由于颌面部的肿瘤、感染、外伤等多种疾病造成，目前临床治疗效果常不确切[]。（剩余12694字）