壳聚糖盐酸盐/磺丁基-β-环糊精纳米载药体系增强CCCH-ZAP抗ALV-J活性研究

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Abstract：CCCH-zinc-fingerantiviral proteincansignificantlyinhibit Javian leukemiavirus infectionandrelieve the immunesuppressioncausedbyJavian leukemiavirus infection.However，theapplicationofproteindrugsislimitedbypoor immunogenicitytresresposecausedbyijectionndlowalbioavailabilityToexporeteationaloraldstation of CCCH-zinc-finger antiviral protein，chitosan hydrochloride and hydrochloride/sulfobutyl- β -cyclodextrin with good water solubility were selected and a systemof ion-crosslinked chitosan hydrochloride/sulfobutyl- ⋅β -cyclodextrinwas constructed to achieve the loading of CCCH-zinc-fingerantiviral protein.Besides，theanti Javian leukemia virus infection virus activity cytotoxicity，andtransmembrane behavior were studied.Resultsshowed thatthesystem hasgoodcellpermeabilityand CCCH-zinc-finger antiviral protein can be loaded successfully，with a loading rate of 61.5% at pH=7.4 After loading，the inhibition rateofCCCH-zinc-finger antiviral protein againstJavian leukemia virus infection was significantly enhanced， with an inhibition rate of 55.0% at 72 hours，and the difference of inhibitory activity enlarged with time.The study indicates that chitosan hydrochloride/sulfobutyl- β -cyclodextrin system is a promising carrier for protein drugs，providing a basis for oral delivery of protein drugs in the prevention of avian leukemia virus.

Keywords： Chitosan; drug loading; CCCH-ZAP; antiviral activity

J亚群禽白血病病毒（AvianleukosisvirussubgroupJ，ALV-J是禽白血病病毒的重要成员，不仅造成鸡的髓细胞性白血病，还会诱导多种肿瘤的发生并造成严重的免疫抑制[1]。（剩余9695字）