棉花根际高抗盐碱芽孢杆菌的筛选鉴定与功能研究

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Abstract：Soilsalinizationisoneofthemajorabioticstressesaffectingglobalagriculturalproduction.InChina，widespread saline-alkalisoilsseverelyrestrictcropyieldandqualityCottn，asanimportanteconomicropinChina，holdsasigniicant positioninthetextileindustryanditsoilandproteinarealsovitalfoodandindustrialrawmaterials.However，creased osmotic pressre，nutrientdeficiencyandsoilstructuredisruptioncausedbysoilsalinizationseverelylimitcotongrowth anddevelopment.Toaddresthischalenge，this studyaims toimprovethephysicochemical propertiesofsaline-alkali soils andthereby enhance theresistanceand yieldofcoton in saline-alkali lands through screening andutilizing Bacilus strains with high salt-alkali resistance fromcotton hizosphere.Our researchteamisolates strains with saline-alkali resistance from cottonhizospheresoilandselectstwmultifunctional，ighlysaline-alkali-resistantBacillusstrainsA3-3ndN-7though progressive salinity-alkalinity acclimation.Strain A3-3 can grow under high saline-alkali conditions of 15% NaCl and pH 9.0，and possessesarious bioactivities，includingiorganicphosphorussolubilzation，rganicphosphorusdecomposition， potassium solubilization，protease production，cellase production，andantagonismagainstcoton wilt disease，preliiarily identified as Bacillus halotolerans.Strain N-7 can growunder high saline-alkali conditions of 15% NaCl and pH11.0， and exhibitssimilarfunctionsasstrainA3-3preliminarilyidentifiedasBacilushaynesi.Potexperimentsshowthatbothstrains significantlypromotecottongrowthundermoderate toseveresaline-alkali stres.Therefore，StrainsA3-3andN-7have great potential inthe developmentandapplicationof coton-specificsaline-alkali-resistant microbial agents，providing new strategies for bioremediation of saline-alkali soils and sustainable cotton production.

Keywords：Cotton; moderate to severe saline-alkali land; saline-alkali-resistant Bacillus; function stud

土壤盐碱化是全球农业发展中面临的关键环境挑战，根据土壤中所含盐浓度及pH，将盐碱胁迫分为三个等级[1：轻度（盐分含量 ⩽0.3% ，pH7.1＼～8.5）、中度（盐分含量 0.3%～0.6% ， pH8.5～ 9.5）和重度（盐分含量 ⩾0.6%，pH9.5） ，目前对于中重度盐碱化土壤的治理难度较大[2]。（剩余17096字）