老秃顶子国家自然保护区2种蛇的肠道微生物组成和特征分析

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中图分类号：Q955 文献标志码：A doi： 10.3969/j .issn.1673-5862.2025.03.007

Analysis of intestinal microbial composition and characteristics of two kinds of snakes in Laotudingzi Nature Reserve

DONG Bingjun，MIAO Ziyuan，LI Zhenxin，SHEN Chao （College of Life Science，ShenyangNormal University，Shenyang1loo34，China）

Abstract： This study focuses on the wild Oocatochus rufodorsatus and Gloydius ussuriensis from the Laotudingzi National Nature Reserve. Five adult individuals of each species were sampled，and samples from different sections of the host intestines（small intestine，large intestine，and cloaca） were mixed to explore the differences and characteristics of the overall intestinal microbial composition between the two snake species. High-throughput sequencing of 16S rDNA revealed no significant differences in both Alpha and Beta diversity of the intestinal microbiota between the two species. The dominant bacterial phyla in O.rufodorsatus were Proteobacteria， Firmicutes， Fusobacteria，Bacteroidetes，and Actinobacteria，while those in G.ussuriensis were Proteobacteria， Firmicutes，Fusobacteria，Bacteroidetes，Actinobacteria，and Cyanobacteria.Notably， the abundance of Bacteroidetes showed significant diferences between the two species. At the genus level，significant differences were observed in Salmonella，Citrobacter，Bacteroides，and

Achromobacter. In conclusion，at both the phylum and genus levels，the intestinal microbial community structuresof O rufodorsatus and G .ussuriensis exhibit high consistency. Additionally，numerous probiotics（e. g.，ul14） and potential pathogens（e. g.，Salmonella） were identified in the intestines of both species，providing data support for understanding the composition of wild intestinal microbiota in these snakes.

Key Words： Oocatochus rufodorsatus ; Gloydius ussurensis ； gut microbiota; high-throughput se quencing

肠道微生物的多样性是微生物群落与宿主之间长期协同进化的结果[1]。（剩余12360字）