异甘草素缓解胺碘酮诱导人脐静脉内皮细胞损伤的实验研究

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Experimental study on the alleviation of amiodarone induced damage to human umbilical vein endothelial cells by isoliquiritigenin

Abstract  Objective：The aim of this study was to observe the protective effect of isoliquiritigenin（ISL） on damaged human umbilical vein endothelial cells（HUVECs） induced by amiodarone（AM）⁃induced venous inflammation.Methods：HUVECs were divided into three groups：control group，AM group（treated with AM at a concentration of 30 μmol/L for 24 h），and ISL group（pretreated with ISL at a concentration of 10 μmol/L for 1 h followed by AM at a concentration of 30 μmol/L treatment for 24 h）.The CCK⁃8 assay was used to determine the effective concentration of ISL.The scratch assay was performed to evaluate cell migration ability.Flow cytometry was used to assess cell apoptosis.Polymerase chain reaction（PCR） was conducted to detect the expression of proliferation factor PCNA and apoptotic factor Casepase⁃3.The expression of inflammatory factors tumor necrosis factor⁃α（TNF⁃α） and interleukin⁃6（IL⁃6），and adhesion factors intercellular cell adhesion molecule⁃1（ICAM⁃1） and vascular cell adhesion molecule⁃1（VCAM⁃1） were measured.Protein levels of ICAM⁃1 and VCAM⁃1 were detected using Western Blot.Results：Compared with the AM group，ISL pretreatment promoted cell proliferation and migration，inhibited cell apoptosis，and downregulated related inflammatory factors.Conclusion：ISL shows promising application prospects in preventing and treating clinical venous inflammation，and can be considered as a potential therapeutic drug for preventing venous inflammation.

Keywords  amiodarone； endothelial cells； isoliquiritigenin； venous inflammation； inflammatory factors； experimental study

摘要  目的：通过胺碘酮诱导静脉炎，观察异甘草素对受损的人脐静脉内皮细胞的保护作用。（剩余13006字）