卡瑞利珠单抗联合顺铂诱导食管鳞癌Eca-109细胞凋亡的研究

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中图分类号：R735.1 文献标志码：A DOI：10.3969/j.issn.1007-7146.2025.03.010

Abstract：Inorder to study the effctofcarilizumabcombined with cisplatinonapoptosis of esophagealsquamous cell carcinoma celsEca-109byregulating mitochondrialoxidative phosphorylation （OXPHOS）andphosphatidylinositol3 kiase/ proteinkinaseB（PI3K/AKT）signaling pathway，andtoobservetheexpresionofheatshock proteinA9（HSPA9）.Inthis study， the test was divided into blank control group，cisplatin group （100μmol/L ）and combined administration group which is cisplatin （100μmol/L）⋅ +carrilizumab 200mg/time ）.Transwell migration test，scratch test，cell counting kit8（CCK-8） test andclonalaggregation technique were used toobserve the effectsofcarrelizumabcombined with cisplatinontheinvasion， migrationand proliferationof esophagealsquamouscellcarcinomacelsEca-19.The expressionofHSPA9gene wasdetected by RT-PCR. Westernblot（WB）was used to detect the expresion ofOXPHOS and PI3K/AKTpathway proteins.Mitochondrial reactiveoxygenspecies （ROS）were detected byflowcytometry.Transwellmigration，scratchtest，CCK-8 testandclonal formationtestshowedthatthecombinedadministrationgroupihbitedtheactivitymigrationandaggregationofEca-O9cells more than the cisplatin group.Reverse transcription-polymerase chain reaction （RT-PCR）and WB detectionresults showed that compared with cisplatin group and control group，the expresion of HSPA9 and OXPHOS in combined administration group significantly decreased，and the phosphorylation of PI3K/AKTwas inhibited （P<0.01 ）. Flow cytometry showed that compared withcontrolgroupandcisplatin group，ROSlevelsincisplatin+carilizumab groupsignificantly increased，andtherewas a significantdifference ⋅P<0.05 ）.Compared with cisplatinalone，carrellizumabcombined with cisplatincould significantly inhibit the expressionofHSPA9andOXPHOS inesophagealsquamous cellcancercells，ihibit thephosphorylationofPI3K/ AKTsignalingpathwayandregulatereoxidationandmitochondrialhomeostasis.TheinhibitionofEca-1O9onesophageal squamous cellcarcinoma by inducing mitochondrial apoptosis provides a reference forthe precise interventionandtreatmentof esophageal squamous cell carcinoma in clinical practice.

Key words： carrilizumab; cisplatin; esophageal squamouscell carcinoma; OXPHOS; HSPA9 （ActaLaser Biology Sinica，2025，34（3）：274-281）

食管癌（esophagealcancer）是临床上发病率较高、恶性程度较高的一类消化道肿瘤，因临床症状较隐匿，早期不容易被及时发现，等出现临床症状时已是中晚期。（剩余12817字）