光谱流式细胞仪在生物材料检测中的应用研究

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中图分类号：Q631 文献标志码：A DOI：10.3969/j.issn.1007-7146.2025.03.004

Abstract：Asadetection instrument developed rapidlyinrecent years，the spectral flowcytometer primarilycomplemented traditionalflowcytometry.Thisarticledemonstratedtheperformanceof threespectralflowcytometers inthedetectionof fluorescent materials，demonstratingtheirpotentialapplications inthefieldofbiological materials.Studies showedthatthe spectral flowcytometercouldnotonlyexplorefluorescencefromnon-primarybandsofknown fluorescent dyes，but also decompose overlapping spectrabetween fluorescentmaterials，andreagentkitdetectionchannels，esulting inmoreaccurate analyticalresultsdditioallyituldtecttesctralsiftsoffuorestomaterialsiinell.Ialltstral flowcytometercouldrecordthespectralcharacteristicsoffluorescentmaterialsenteringcels，analyethespectralbehaviorof fluorophores within cells，and spectrallyresolve fluorescence signals that overlapped with detection channels，thus providing objective fluorescence data.These capabilities opened up new paths for the research offluorescent materials，providing theoreticalfoundationsaswellaspracticalreferencesforthewidespreaduseofspectralflowcyometryinbiomaterialsscience

Key words： spectral flow cytometry; biological materials; fluorescent materials; fluorescence spectrum shift;spectral separation

（ActaLaser BiologySinica，2025，34（3）：221-228）

近年来，流式细胞仪及流式细胞术发展迅猛，研究人员创新研究了多种类型的流式细胞仪，包括光谱流式细胞仪（spectrumflowcytometry）、质谱流式细胞仪（masscytometry）、成像流式细胞仪（imagingcytometry）以及组织成像质谱流式细胞仪（imagingmasscytometry）等。（剩余12814字）