miR-133a低表达对高糖诱导足细胞炎症凋亡的改善作用及相关机制研究
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中图分类号：R364.5 文献标志码：A DOI：10.3969/j.issn.1007-7146.2025.03.009
Abstract：To investigate theefectoflow expressonofmicroRNAs-133a（miR-133a）ontheapoptosisofpodocytes induced byhighglucoseanditsrelatedmechanism，humanglomerularpodocyte （HGPC）celswerecultured invitronddividedinto control group（Con，notreatment），HGgroup（3Ommol/LD-glucose），ibitorNCgroup（HGgroup+transfectioninbitor NC）， miR-133a inbitor group （HGgroup+transfectionmiR-133a inhibitor）.24hafter ransfectionreal-timefluorescence quantitativePCR（RT-qPCR）was usedto detectthe transfection effciency.Follow-uptests were performedafter transfection success， which were divided into Con group，HG group，inhibitorNCgroupand miR-133ainhibitor group，miR-133a inhibi tor+phosphatidylinositol3-kinase（PI3K）/protein kinaseB（AKT）pathway inhibitor group （miR-133ainhibitol +10μmol/L （20
LY294002），miR-133a inhibitor + PI3K/AKT pathway activator group [miR-133a inhibitor +50mg/mL insulin-like growth factor （IGF-1）].Livecell counting method（CCK-8）and Hoechst33258 staining wereusedrespectively，enzyme-linked immunosorbentassy（ELISA）andWestemblotwereusedtoetectHGPCcellviabilityapoptosisate，tumornecrosisactor （TNF）-α，interleukin-6（IL-6）levels，PI3K，phosphorylation（p）-I3K，AKTandp-AKTexpressionlevels.miR-133aas transfected successfully. The cell viability，p-PI3K and p -AKT protein expression of HG group and inhibitor NC group were lower than those of Con group （P<0.05 ），theapoptosisrate，NF ∇⋅a and IL-6 levels were higher than those of Con group （P<0.05 ）.Theapoptosisrate，TNF- ∇⋅a and IL-6 levels of miR-133a inhibitor group were lower than those of miR-133a inhibitor group ），while the protein expressions of p -PI3K and p-AKT increased （ ）.LY294002 attenuated the action ofmiR-133ainhibitorandIGF-IenhancedtheactionofmiR-133ainhibitor.ThelowexpresionofmiR-133acanregulatethe PI3K/AKTpathwaytoinhibitthereleaseofinflammatoryfactorsandapoptosisofHG-inducedHGPCcels，tisresearchcould provideanew direction for the study of diseases related to diabetic nephropathy.
Key words： diabetic nephropathy; miR-133a; HGPC cels; high glucose; inflammation （ActaLaserBiology Sinica，2025，34（3）：267-273）
中国糖尿病（diabetes）患者众多，也是世界上糖尿病患病率增长较快的国家，在2021年糖尿病患病人数已经达到1.4亿[1]。（剩余12100字）