酵母单杂与亚细胞定位技术在水稻抗病基因启动子互作蛋白筛选中的应用进展

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中图分类号：S-03 文献标志码：A 文章编号：1673-6737（2025）03-0031-05

Abstract：Asanimportantglobalfoodcrop，diseasemanagementofriceisof greatsignificance forensuringfoodsecurity. Theresearchaims toreview theapplication progressofyeastone hybridandsubcelularlocalization techniquesin screningpromoterinteractingproteinsofricediseaseresistancegenes，andexplorethepotentialofthesetechniquesin improvingricediseaseresistance.Byanalyzing theapplicationcasesof these technologies，guidancewillbe provided for futureresearchdirections，andnewstrategiesandmethodswillbeprovidedforricediseaseresistancebreding.The resultsindicatethatyeastonehybridtechnologyhashighsensivityandsimpleoperationinidentifyingspecificbinding proteinsof specificDNA sequences，whilesubcelular localizationtechnologyprovidesan important perspectivefor understanding protein function and its role in signal transduction pathways.

KeyWords：Rice;Disease-resistant genes；Golden iron lock；Yeast singlecompound;Subcelularlocalization

在现代农业生产中，水稻作为全球重要的粮食作物之一，其病害管理尤为关键。（剩余7403字）