兼养培养对淡水微藻胞内活性物质的影响

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中图分类号：S917.3 文献标志码：A

Effects of Mixotrophy on Intracellular Metabolite of Microalgaes in Freshwater

FANG Xia 1，2 ， XIA Wanyue 2 ，SUN Lu 2 ， PHUNG Nghi Van²，LI Chen 2 ，DONG Bingzhe 2 FAN Yong²，LI Qiang'，LI Fuli²

（1.School of Biological Science and Technology，Universityof Jinan，Jinan 25OO22，Shandong，China; 2.Qingdao Instituteof BioenergyandBioprocss Technology，Chinese Academyof Sciences，Qingdao ，Shandong，China）

Abstract：Toinvestigate thediferencesingrowthandintracelular metaboliteaccumulationofMychonastes@fer（M.afer） underariousconditions，theeconomicallyimportantalgal species Chlorellsorokiniana（C.sorokiniana）inaquaculture wasusedasacontrol，the growth，organiccarbon sources，pigmentcompositionand content，antioxidantcapacity，and fatyacid composition of thetwo microalgaes wereevaluated under both autotrophic and mixotrophicconditions.The results show that both M .afer and C .sorokiniana exhibit accelerated growth in mixotrophic conditions.M. afer demonstrates an elevated pigment content and antioxidant capacity，showing a 504% increase in chlorophyll a，a 149% increase inchlorophyll b ，and an 88% increase in carotenoid content compared to autotrophic conditions，while the pigment content and antioxidant capacity of C .sorokiniana decrease compared to autotrophic conditions. The proportion of saturated fatty acids and monounsaturated fatty acids in M .afer also increase considerably，and the content of nervonic acid C24：1 increases by 145% . The two microalgaes exhibit significant differences in their synergistic metabolic mechanisms of light energyandorganic carbonsources.Under mixotrophicconditions，M.aferdemonstrates higher productionrates and greater contents of active substances.

Keywords：mixotrophy；Mychonastes afer；Chlorella sorokiniana；intracellular metabolite

微藻是水生环境中普遍存在的光合微生物，所有水生环境几乎都有分布，包括淡水、海水以及潮湿的陆地栖息地。（剩余15812字）