高效液相色谱法同时测定麻仁胶囊中10种成分的含量

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中图分类号：R284.1 文献标志码：A 文章编号：1003-9783（2025）09-1566-07

doi：10.19378/j.issn.1003-9783.2025.09.015

Simultaneous Determination of Ten Components in Maren Capsules by HPLC

HE Yan1 ，HU Xiaoxiang²（1.College of Pharmacy，Xiang Nan University，Chenzhou 423099 Hunan，China；2.Cher Zhou Municipal Center for Food and Drug Control，Chenzhou 423OOo Hunan，China ）

Abstract：Objective To establish a high-performance liquid chromatography（HPLC）method for the simultaneous determination of ten components in Maren Capsules，including paeoniflorin，hesperidin，aloe-emodin，nobiletin， rhein，emodin，honokiol，magnolol，chrysophanol，and physcion.Methods The analysis was performed on a Pntulips B NT-C column （ 250mm×4.6mm ， 5μm ）maintained at 30∘C .The mobile phase consisted of acetonitrile 18 and- 0.1% phosphoric acid with gradient elution at a flow rate of 0.9mL⋅min-1 . The detection wavelength was set at （20 230nm ，and the injection volume was 10μL .Results The ten components exhibited good linear relationships within their respective concentration ranges （ r⩾0.9993 ）. The average recoveries were 97.82% ， 99.28% ， 97.83% ， 100.57% ， 101.80% ， 98.28% ， 99.09% ， 102.66% ， 101.71% ，and 101.30% ，with RSD values all below 3.0% . In six batches of Maren Capsules，the average content ranges of paeoniflorin， hesperidin，aloe-emodin，nobiletin，rhein，emodin， honokiol，magnolol，chrysophanol，and physcion were 5.6771-6.9871，1.2609-2.7185，0.5103-0.8032，0.1628- 0.2757，0.170 1-0.2623，0.3071-0.7478，0.058 7-0.0757，0.1013-0.1547，2.8629-9.2512，and 0.564 6- （204号 1.3464mg⋅g-1 ，respectively.Conclusion This method isaccurate and reproducible，suitable for the quality evaluation and control of Maren capsules，and provides a reference for improving its quality standards .

Keywords：Maren Capsules ；high-performance liquid chromatography（HPLC）；content determination；quality control

麻仁胶囊是由火麻仁、苦香仁、熟大黄、枳实（炒）、厚朴（姜制）、白芍（炒）6味中药经一定的生产工艺加工而成的中药复方制剂，其功效为润肠通便，临床常用于治疗肠燥便秘等症，效果显著[-2]。（剩余7529字）