光声光谱二氧化碳传感的种子活力检测

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loi:10.37188/OPE.20253303.0367 CSTR:32169.14.OPE.20253303.0367
Detection of seed viability by photoacoustic carbon dioxide sensing
GUO Zhenyu,FAN Yashan,ZHAI Baojie,XIE Ruijun,SHANG Zhijin,TIAN Yali,QIU Xuanbing*,LI Chuanliang
(Shanxi Precision Measurement and Online Testing Equipment Engineering Research Center, College of Applied Science, Taiyuan University ofScience and Technology, Taiyuan O3OO24,China) * Corresponding author, E -mail: qiuxb@tyust. edu. cn
Abstract: In order to investigate the relationship between variations in carbon dioxide ( CO2 )concentration and seed vitality during respiration,a CO 2 photoacoustic sensing system utilizing a differential Helmholtz photoacoustic cell was designed. The system's performance was evaluated by measuring the CO2 production rate of south japonica rice seeds exhibiting varying levels of vitality. An independent signal processing module based on a field programmable gate array (FPGA)development board was developed to facilitate precise regulation of laser drive current and demodulation of photoacoustic signals. Through optimization of modulation frequency,laser modulation depth,working pressure,and other system parameters, optimal performance was achieved,with an integration time of 1 O89 s and a detection limit of 0.6×10-6 :
In accordance with national standard GB/T 3543.1,a germination experiment involving 30g of south japon ica rice seeds was conducted.Linear fitting analysis was performed using CO2 evolution concentration data obtained from the photoacoustic sensing experiment. The results demonstrate that the seed respiration rate of south japonica rice is directly proportional to seed vitality,with a correlation coefficient of O.979.This finding provides a valuable reference for assessng rice seed vitality through photoacoustic spectroscopy.
Key words: photoacoustic spectroscopy;seed viability test; Helmholtz photoacoustic cell;carbon dioxide
1引言
种子活力是指种子在特定环境条件下表现出的生长和发育能力,反映种子在萌发过程中所表现出的生命力和健康状况[1。(剩余14564字)