从拉伸到信号：YAP1和PIEZO2在膀胱尿路上皮细胞感知中的作用

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ABSTRACT：ObjectiveTo explore the roles of the mechanoreceptor Yes-associated protein l（YAPl）and piezo type mechanosensitive ion channel component 2（PIEZO2）in mechanotransduction in mouse bladder urothelial cels.Methods Mousebladderurothelialcellsweresubjected tomecanicalstretchingusing theFX-6oTcellstretching systemandtreated with the YAPl-specific inhibitor verteporfin （VP）.The expressions of PIEZO2，YAPl and connective tissue growth factor （CTGF）atthemRNA and protein levels，as wellas changes incelular adenosine triphosphatase（ATP）concentration，were detected using reverse transcription quantitative PCR（RT-qPCR）and Western blotting（WB）.ResultsAfter stretching stimulation，under the fluorescence microscope，itwas observedthatthediameterlengthofthestretchedcels werelongerthan that before stretching，and the difference was statistically significant （ .The expressions ofYAP1，PIEZO2 and CTGF atthe mRNAand protein levels were increased in the stretched group compared to those of the non-stretched group（ P< 0.05）.VP efectively reduced the expresions of YAP1，PIEZO2 and CTGF at the mRNA and protein levels after stretching stimulation （ P<0.05 . Stretching stimulation significantly increased the intracellular ATP concentration，while VP was able to inhibit the increase in ATP concentration，with a statistically significant difference （ P<0.000 1） .ConclusionStretching stimulationincreased theexpressions of YAPland PIEZO2 inbladderurothelialcellsand promoted thereleaseof ATP; verteporfininhibitedtheincreaseinYAPlactivityandtheoverexpresionof PIEZO2causedbystretching，therebyreducingthe releaseof ATP.It is suggsted that mousebladder urothelial celsmay primarilysense mechanical signals through theYAPlPIEZO2-ATP pathway.

KEY WORDS：bladderurothelialcels；mechanical signal transduction；piezo type mechanosensitive ion channelcomponent; Yes-associated protein l ;connective tissue growth factor

摘要：目的探讨机械感受器Yes相关蛋白1（YAP1）和压电型机械敏感离子通道蛋白2（PIEZO2）在小鼠膀胱尿路上皮细胞机械信号转导中的作用机制。（剩余10858字）