Apelin通过激活FGF2/FGFR1通路促进膀胱癌增殖、迁移及血管生成

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Abstract：ObjectiveToinvestigatetheoleofapelininegulatingproliferationmigrationandangiogenesisofbladerancer cellsand theposibleregulatorymechanism.MethodsGEOdatabasewasusedtoscreenthediferentiallyexpressedgenes in bladdercancertissues andcells.Bladdercancerandpairedadjacenttissues werecollectedfrom 60 patients foranalysis of apelinexpressionsinrelationtoclinicopathologicalparameters.InculturedbladdercancerJ82celsandumanumbilicalein endothelialcels （HUVECs），theeffectsof transfection withan apelin-overexpressing plasmid orspecificsiRNAs targeting apelin，fibroblastgrowthfactor2（FGF2）andfibroblastgrowthactorreceptor1（FGFR1）onproliferationandmigratioofJ82 cellsandtubetioinUeeadsingateongssayaellsydiogesi changes inFGF2expressionandFGFR1phosphorylationweredetectedusing Westernbloting.ResultsTheexpressionlevelof apelinwassignificantlygherinbadderancertissuestnadjacenttissueandbaddercancercelines（24andJ）also expresed highermRNAand protein levelsof apelinthan SV-HUC-1cels.Apelin expressonlevel inbladdercancer tissues was correlated withtumor inasion，distantmetastasisandadvancedTMstages.Apelinknockdownsignificantlysuessed proliferationandmigrationofJ82celsand decreasedthetotal angiogenic lengthofHUVECs.Incontrast，apelin overexpression significantlypromoted proliferation and migrationand enhancedFGFR1phosphorylation inJ82cels，and increasedthetotalangiogensislengthinUVECsuttisectswereectivelyigatedbytrasfetionoftelswith FGF2 siRNAorFGFR1 siRNA.Conclusion Highexpresionof apelin promotes J82cellproliferationandmigrationand HUVEC angiogenesis by promoting activation of the FGF2/FGFR1 pathway.

Keywords： bladder cancer; apelin; FGF2/FGFR1 pathway; J82 cells; angiogenesis

膀胱癌（BC）是一种常见的泌尿系统恶性肿瘤，其发生率和死亡率都很高。（剩余13165字）