神经特异性跨膜蛋白240与过氧化物酶体共定位并激活RhoGDP解离抑制因子β

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Abstract：ObjectiveToinvestigatethesubcelllarlocalizationandbiological functionsof transmembraneprotein240 （TMEM240）. Methods NCBI BLAST and TMHMM bioinformatics software were used for protein sequence analysis and predictionof transmembranedomainofTMEM240.BraintissesfrommaleC57BL/6mice（18-20daysold）wereexamined for distributionofTMEM240usinginsituhybridization，andqPCRandWesternbloting wereusedtodetectTMEM240 expressionin different mouse tissuesand incortical neuronsatdifferent timepoints （n=3） .Intheinitroexperiment，HeG2 andNeuro-2acellsweretransfectedwithplasmidsforoverexpressonofTMEM240andsubcellarlocalizationofM240 Was analyzed using cellimaging In primary cultures of cortical neurons isolated from C57BL/6 mice，TMEM240 expression anditsbiological functionswereinvestigatedusingqPCR，Westernblotingandimmunofluorescencestaining.esults Humanandmouse TMEM240proteinssharea 97.69% similarityintheproteinsequences，andbotharetransmembrane proteinswithtwotransmembranedomains.TMEM240mRNAandproteinwerehighlyexpressedinmousebraintissuesand cortical neurons.Inisolated mouse corticalneurons，TMEM240 expresionreached the peak levelafter primaryculture for9 daysanddistributedinscateredspotswithinthecels.InHepG2cells，TMEM240wascharacterizedasintracelular membrane structures and showed 80% colocalization with peroxisomes. In Neuro-2a cells， TMEM240 overexpression caused significant enhancement of the expressions of Rho GDP dissociation inhibitor β （ARHGDIB） at both the mRNA and protein levels.ConclusionTMEM240isanovel intracellarsubcellularstructure specificallyexpressed inneurons withsignificant potential for targeted cellular function regulation.

Keywords：TMEM240; neurons;subcellularlocalization;peroxisomes;RhoGDPdissociation inhibitor

尽管现在已经确定了人类基因组的整个序列，但是许多基因的功能仍然知之甚少，它们的作用机理尚不清楚。（剩余16862字）