肾透明细胞癌患者的免疫细胞浸润特征与临床病理参数具有相关性

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Abstract：ObjectiveToinvestigatethecharacteristicsofimmunecellinfiltrationintumorsamplesfrom Chinesepatientswith clearcellrenalcellcarcinoma （ccRCC）andthecorelationofimmunecellinfiltrationwithtumor stageandresponseto immunotherapy.MethodsTumorsamplesandClinicopathologicaldatawerecolectedfrom154ccRCCpatientstreatedin NanfangHospital，thialUesirotobettobe3.eelltsii tumortissues wereidentifiedusingimmunohistochemistryandimmunofluorescencestainingandtheircorrelationswiththe patients'clinicopathologicalcharacteristicswereanalyzed.Patient-derivedtumortisuefragmentmodels（PDTF）models， constructedusingtumortisuesfrom22patients，weretreatedwithPD-1monoclonalantibodyandTcellactivationwas detectedusing flowcytometrytoassessthepatients'responses toimmunotherapy.ResultsInChineseccRCCpatients included inthisstudy，CD8*Tcells，CD4*Tcells，and CD3Tcellswerethe mostabundantinthe tumor tisues.Higher infiltration levelsof CD3+ T cells （P=0.004）， PD-1+ T cells （P=0.020），CD68+ T cells （P=0.049）， CD79+ T cells （P=0.049），and Tryptase+cells （P=0.049）were all positively correlated with a larger tumor size （25cm ）.Ahigher infiltrationlevelof CD4T cellswasassociatedwithalower tumorstage.Patients withhigher InternationalSocietyofUrologicalPathology（SUP）grades hadhigherinfiltrationlevelsofCD3+Tcells （P=0.023） ！ CD8+ Tcells （P=0.045） ，PD ⋅1+ T cells （P=0.014） 中 CD20+ B cells （P=0.020） and CD79+ B cells （P=0.049），and lower levels of Tryptase* cells （P=0.001） . Patients with abundant infiltrating immune cells tendedtohavebeterresponses toimmunotherapy.ConclusionTheinfiltratingimmunecellareheterogeneousinChinese ccRCCpatientsandimunecellinfitrationcharacteristcsaecoselyorrelatedwithcliioathologicalparamersofthe patients.

Keywords： clearcellrenal cellcarcinoma; tumor-infiltratinglymphocytes; patient-derived tumor tissefragmentmodel：

肾透明细胞癌（ccRCC）起源于近端肾小管的上皮细胞，是成人中最常见的肾细胞癌（RCC）类型，约占所有肾细胞癌病例的 80%11，2] 。（剩余14813字）