基于生物碱类成分结合多元统计分析评价甘肃不同产地栽培半夏药材的质量特征

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中图分类号:S567 文献标识码:A 文章编号:0488-5368(2025)04-0036-09

Abstract:Multivariate statistical method was used to analyze the content characteristics of nine alkaloids in Pinelia ternata cultivated in different regions of Gansu province,and the relationship between alkaloid content and geographical origin was explored.High-performance liquid chromatography(HPLC)was used to determin theconcentrationsofnine alkaloids,including trigonelline,nicotine,5’inosinic acid,uridine,adenosine,inosine, vernine,thymidine and ephedrine in 39 pinelia samples from four main producing areas in Gansu Province.Non - parametric test,corrlation analysis,principal component analysis,cluster analysis and discriminant analysis wereused to analyze and evaluate therelationship between alkaloid content and geographical origin.The results showed that,exceptfor thecontentof5'Inosinicacid,which follwedanormal distribution,theother eightalkaloids were skewed.Thecontents of nicotine,trigoneline,uridine,and 5'inosinicacid were relatively higher than thoseof the other alkaloids..The Kruskal Wallis test showed significant diferences in alkaloid content among Pinellia ternata samples from different producing areas ( P < 0 . 0 5 ). The alkaloid content was higher in Qinzhou Districtand Xihe County.Correlation analysis revealed complex interrelationships among alkaloid components. Using a eigenvalue greater than 1 as the criterion,three principal components were extracted through principal component analysis,with a cumulative variance contribution rate of 7 5 . 2 % . The results of cluster analysis were largely consistent with those of the Kruskal-Walis test and principal component analysis.The results of OPLSDA analysis and principal component analysis mutually confirmed that nicotine,trigonelline,uridine,and -inosinic acid were the main characteristic alkaloid components of Pinelia ternata.In conclusion,the alkaloid quality characteristics of pinellia pinella from different producing areas in Gansu province exhibit significant diffrences. This study provides scientific basis for identification,quality controland highquality developmentof pinelia from Gansu Province.

Key words:Pinellia ternata ; Gansu ; Diffrent producing areas;Alkaloid;Multivariate statistical analysis

半夏(Pinelliaternata(Thunb.)Breit.),别名蝎子草、麻玉果,是天南星科植物半夏的干燥块茎。(剩余10080字)

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