噬几丁质属菌株ZLX3产生广谱抗菌素的发酵条件优化

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中图分类号：TQ927 文献标志码：A

Abstract：ObjectiveTosreentheoptiumfermentationconditionsforproducingbroad-spectrumantibioticsbythestrainChiinopha ga sp.ZLX 3.Methods The method of filter paper was used for drug susceptibility test.The optimal pH value，the optimal yeast extractconcentration，teoptimalpeptoneconcentration，theoptimalculturetie，theotialculturetemperatureandtheptimalspeed ofthefermentationmediumofstrainZLX3producingantibioticswerescreenedbysinglefactormethodThethreemostsignificantinfluencingfactorswereobtainedbySPSSsoftware.InDesign-Expert，Box-Behnken（responsesurfacemethod）wasusedtocayoutthe experimentaldsignofteteesignifcantiueningfactorsndteinteractiondegreeoftethsignificantifluencingfctorson theantimicrobialactivityproduedbystrainZLX3wasobtained.ThefitingmultiplequadraticregresionequationshowedthefunctionalrelatioshipetweenthetreefementationconditionsandtheantibacterialactivityfthemetabliesofstrainZLX3，andte optimalfitingvueoffermentationconditionsascalculated.Finally，thefitingvalueswereverifiedbyexperimentstoconfithe optimalfermentationconditionsfortheproductionofantibioticsbystrainZLX3.ResultsThedrugsensitivitytestshowedthatthediameteroftheibiionzoneofmetabolisofeoptimiedstrainZLX3was1.32timesthatofbeforeoptimization，dicatinganiease intheantibacterialatiyofmetabolitesintheoptiizedstrainZLX3.ConclusionTheoptimalfermentationconditionsforhepro duction of antibiotics by strain ZLX 3 were pH7.2 ， 1g⋅L-1 of yeast extract， 30g⋅L-1 of peptone， 25.2‰ ， and 4 d. This studylaidafoundationfortelatersdyftehyscochemicalpropeiesntibacterialmchanismndlarge-scaleproductiofthe antibiotic produced by strain ZLX 3. Key Words： Chitinophaga sp. ;the strain ZLX 3;broad-spectrum antibiotics;fermentation condition;optimize

自从以青霉素为代表的抗生素应用于临床以来，抗生素挽救了无数人和动物的生命。（剩余13541字）