海洋真菌Trichodermasp.MDCW-16次级代谢产物的研究

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中图分类号：R978 文献标志码：A

Study on secondary metabolites of marine fungus Trichoderma sp.MDCW-16

Xu Rui1， Xu Xinyan1， Xu Huil， Chen Minjun1，Yang Xuefang1，Li Peihai²， Kong Fandong1，and Wang Cong1 （1KeyLaboratoryofChemistryandEngeeringofForestProducts，StateEthnicAfairsCommission;GuangxiKeyLaboratoryof

ChemistryandEngineringofForestProducts/GuangxiColaborativeInnovationCenterforChemistryandEngineringofForestProucts，

GuangxiMinzuUniversityNanning3ooo;EngineeringResearchCenterofZbrafishodelsforHumanDiseasesandDrugreein of ShandongProvine，iologyIstitute，QiluUniversityofechnology（ShandongcademyofSiences），Jian50103）

Abstract Objective To extract the secondary metabolites produced by Trichoderma sp. MDCW-16 from Beibu Gulf and banana wiltcaused by Fusarium oxysporum and study their activities.MethodsThe isolation and purification of compounds were performed by means of column chromatography. And their structures were elucidated through the analysis ofUV，MS and NMR.The isolatedcompounds were evaluated fortheir proangiogenic activity in zebrafish.ResultsOne new pyranone compound and 9 known compounds were isolated and identified： 4-hydroxyphenylacetic acid methyl ester （2），acetoxyphenylethanol （3），4-hydroxybenzaldehyde （4），2-hydroxy3-phenylpropionate methylester （5）， 4-hydroxyphenethyl-2-（4-hydroxyphenyl）acetate （6）， penilactone D （7），

2-（4-hydroxyphenyl）-ethanol （8）， 6-（（2S，3S）-2，3-dihydroxybutan-2-yl）-3-methyl- 2H⋅ -pyran-2-one （9），6-（（2R，3S）- 2，3-dihydroxybutan-2-yl）-3-methyl- ⋅2H. -pyran-2-one （10）. Compounds 5 and 8 promoted the growth of zebrafish intersegmental vessels at the concentrations of 10 and 20μmol/L ，respectively. Conclusion Compound 1 was proved to be a new pyranone compound. Compounds 5 and 8 had proangiogenic activity.

KeywordsMarine fungi; Trichoderma sp.MDCW-16; Secondary metabolites

海洋环境具有高压、高盐度以及低营养等特点，海洋真菌具有复杂的遗传体系，这使它们能够产生一些与陆地生物不同的活性天然产物。（剩余12271字）