土壤来源拟盾壳霉属真菌Paraconiothyriumsp.ACC31771的次级代谢产物研究

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Studies on the secondary metabolites of the soil-derived peltate fungus Paraconiothyrium sp. ACC31771

Zheng Shengyan1²2，He Liming²，Wang Cong1，Tao Weixin²， Zhang Keyong^，and Cai Yousheng²

（1GuangxiCollborativeInnovationCenterforChemistryandEngeeingofForestProducts，GuangxiKeyLaboratoryofCeisty

andEngineringofFrestProducts，KeyabratoryofemistrynEngieeingofForestProucts，tateEticiso SchoolofChemistrydeicalEngiiguangxiinuUvesityng30o6;hlofaceuticalic， Wuhan University，Wuhan 430o71;3DayePeople'sHospital，Huangshi 435100）

Abstract Objective For searching bioactive secondary metabolites from fungal sources， the fungus Paraconiothyrium sp.ACC31771 isolated from the soil of Kanas Lake，Burjin County， Xinjiang， China， was chemically investigated. MethodsThe soil-erived fungus Paraconiothyrium sp.ACC31771 was fermented using potato dextrose broth （PDB） medium. The fermentation broth and the mycelia were separated by filtration. The mycelia were further extracted by ultrasound with 100% acetone，and then concentrated under reduced pressure to obtain crude extract. Allcompounds were isolated and purified sequentially using silica gel column chromatography， Sephadex LH20 gel column chromatography and semi-preparative liquid chromatography. ResultsSeven known compounds were found and studied.These included decaturin B（1），15-hydroxydecaturin A （2）and oxalicine B （3）， which wereoxalicine-typealkaloids，and1，8-dihydroxy-3-methy1-9，10-anthracenedione （4），1，3，8-trihydroxy-6- methyl-9，10-anthracenedione （5）， citreorosein （6）， and （+） -2'S-isorhodoptilometrin （7）， which were anthracenediones. Compounds 5 and 7 showed inhibitory activity against Staphylococcus aureus with both MIC values of 64μg/mL ：

Key WordsSoil-derived fungi; Paraconiothyrium sp.; Structural identification; Antibacterial activity

喀纳斯湖位于新疆布尔津县，地处较高纬度并具有层状地貌的喀纳斯山区，气候极端，植被复杂，其周围表层土壤有机质的含量随海拔的升高而增加。（剩余13495字）