盐田来源极端嗜盐真菌FusariumincarnatumGXIMD00527抗污损活性成分研究

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Studies on anti-fouling compounds from saltern-derived extreme halophilic fungus Fusarium incarnatum GXIMD00527

LiangLifen，LuChunju，Fu Chunqing，Huang Huaqing，Chen Chunxiao，Qi Mengfan，Luo Xiaowei， Gao Chenghai Liu Yonghong， and Xu Xinya （InstituteofMarineDrugs/Guangxi KeyLaboratoryofMarineDrugs，GuangxiUniversityofChineseMedicine，Nanming 530200）

Abstract Objective To study the secondary metabolites of an extreme halophilic fungus，Fusarium incarnatum GXIMDoo527，and their marine antifouling activities against Amphibalanus amphitrite.MethodsThe secondary metabolites were isolatedby silicagel column chromatographyand purified using high-performance liquid chromatography.Their chemical structures were elucidated bythe comparisons of nuclear magnetic resonance data with literature.Theanti-fouling activities and cytotoxicities ofallcompounds against barnacle larvae atachment were determined.ResultsEight compounds were isolated and identified as four tetramicacid alkaloids： decalintetracid A（1）， decalintetracid B（2）， equisetin（3）， 5′ -epiequisetin（4），and four 14-membered resorcylic acid lactones： zearalenone（5）， a -Zearalenol（6），5'-hydroxyzearalenol（7）， 8'-hydroxyzearalenone（8）. Compounds 1＼～5and 8 exhibited potent inhibitory efectson barnacle larval attchment，and compounds 1，4and 7 showed moderate inhibitory activities on the human colon cancer celline SW480.Conclusion The secondary metabolites of the extreme halophile fungus F. incarnatum GXIMDoo527 were significantly resistant to barnacle attchment fouling. This study firstly reported on anti-fouling compounds from the extreme halophile fungi derived from the Beibu Gulf saltern， which provided a theoretical basis for the development of natural anti-fouling agents.

Key WordsMarine fungus;Fusarium incarnatum; Secondary metabolites; Anti-fouling;Extreme halophilic fungus

海洋生物污损包括微型生物污损（细菌、真菌、微藻等污损微生物附着）和大型生物污损（藤壶、草苔虫、管虫、大型藻类等污损生物附着），对全球生态和经济造成重大影响。（剩余15877字）