1株广西红树林放线菌Streptomycessp.的次级代谢产物及抗菌活性研究

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Study on the secondary metabolites and antimicrobial activity of a Streptomyces sp. isolated from Guangxi mangrove

YangShuyul，Xiao Yang²， Zhang Tangjie²，Liu Mi2 ，Liu Hongcun²，Yang Lifang²，andJiang Mingguo1

（1GuangxiKeyLboatoryoolyaccaideaterialsddifcatio，holofrineiencsadtecologyGagiinzu

University8;lofstlgagiuUetKeboatyt

andEnginergofFrestrodctsStateEtncfisCommsioangxiKeyLboratoryofemistrndEngieegoot

Products，GuangxiCollborativovatioCenterforhemstryandEgieeringofForestProuctsducationepartmentofGuagxi Zhuang Autonomous Region， Nanning 530006）

Abstract Objective To obtain more compounds with significant biological activity，this study investigated the secondary metabolitesand antimicrobial propertiesofa strain of Streptomyces sp. GY-10129，derived from the rhizosphere soil of mangrove Acanthus ilicifolius L.in Guangxi.MethodsTechniques such as silica gel column chromatography， high-performance liquid chromatography，and semi-preparative liquid chromatography were employed to isolate the secondary metabolites of this strain; spectroscopic methods were utilized for the structural identificationof theisolatedcompounds，and thefilterpaper method wasused toasesstheantimicrobialactivityof the individualcompounds.ResultsA total of 10 individualcompounds were successfull isolated from the fermentation products of Streptomyces sp. GY-10129，including 5-hydroxymethylfuran-3-carboxylic acid（1）， phevalin（2）， 4-hydroxybenzaldehyde（3），germicidinA（4），indole-3-acetamide（5），6-methoxy--（-phenylethyl）chromone（6），tras3，4，5-trimethoxy- .4′ -isopentenyloxyl-stilbene（7），1，3-benzodioxole derivative（8）， cycloheximide（9），and 2-methyl4[3H] quinazolinone（10）.compounds1，6and7were discovered in this actinomycete for the first time.The results of biologicalactivitytests showedthatcompound1hadastrong inibitory efectonStaphylococcus aureus.Compounds1and 9 showed weak antibacterial activity against Fusarium pseudograminearum. Conclusion The mangrove actinomycete Streptomyces sp. GY-10129 had the potential research value of producing antimicrobial lead compounds.

Key WordsMangroves; Actinomyces; Separationand purification; Secondarymetabolites;Antibacterialactivity

放线菌（Actinomycetes）是一类胞嘧啶和鸟嘌呤含量高的革兰阳性需氧型细菌[1]，同时也是产生生物活性物质最多的微生物群。（剩余15375字）