基于BSA-seq的高粱柄伸长度QTL定位

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Abstract：[Objective]Thisstudy aimed to identify quantitativetrait loci（QTL）asociated with sorghum peduncle length using BSA-seq technology，screen sorghum plants with excellent peduncle elongation traits，and provideatheoretical basis for improving elite sorghum backbone lines and breding mechanization-adapted varieties.[Methods]The studyused sorghum maintainer lines 7O5OB（short peduncle）and Tx2925B（long peduncle），along with their F 2 population derived from emasculated hybridization.Peduncle length was measured in parents and thesegregating population.Two bulked pools，EL-L（long peduncle）andEL-S（shortpeduncle），wereconstructed forQTLmapping.[Results]Thepeduncle lengthof 7O50Bwassignificantly shorter than that of Tx2925B ，with a phenotypic variation coeficient variation of 76.1% in the F2 population. SNP variations detectedinEL-L，EL-S，7O5OB，and Tx2925B were1574836，2041538，2512 953and688446 respectively.Ata 99% confidencelevel，16QTLswereidentifiedonchromosomes1，4，5，8and1O，including9onchromosome1，4onchromo some4，andleachonchromosomes5，8and10.Thecandidate intervalscontained2 to1，40lgenes，with 2748and1779 transcriptsannotated inGOandKEGGdatabases，respectively.[Conclusion]Thisstudy identified16QTLssignificantlyasso ciated with peduncle length across five sorghum chromosomes，providing candidate gene resources for deciphering the genetic

mechanismsofpeduncleelongation.Thesefindingsestablishamolecularfoundation forbreeding mechanized sorghumvarieties and improving elite germplasms.

Keywords：Sorghum，Peduncle Elongation Length，Genetic Analysis，QTL

高粱作为全球第5大禾谷类作物，在食品、饲料、酿造等行业应用广泛[1]。（剩余13997字）