谷子HMA基因家族的全基因组表征及其功能预测

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中图分类号：S515 文献标识码：A 文章编号：1671-8151（2025）04-0003-13

Abstract：[Objective]Heavy metalATPase（HMA）proteinsplaycriticalroles intheabsorptionand transportofvarious metal ions nplants.Thisstudyaimedtocharacterize theHMAgenefamilyinfoxtailmilletthroughgenomecharacterization，xpres sionprofiling，and homologyanalysis toelucidateitsfunctions.[Method]HMAgenefamilymembers infoxtail millet were identifiedusingproteinhomologyalignmentandconserveddomainscrening.Bioinformaticsanalyses ereperformedtoexaminetheirphysicochemicalproperties，genefamilyexpansion，conserved motifs，phylogeneticrelationships，andproteinmolecu lardocking.Transeriptomic andqRT-PCRanalysesunder CdandFe stresstreatments were conducted toasess metal-responsiveexpressionpatters.Functional predictions forselected SiHMA were inferred through homology with rice.[Results]

Twelve HMA genes were identified and classified into two subgroups.Family expansion involved six HMA genes through two tandem duplication events （four genes，33. 3% ） and one segmental duplication event （two genes，16. 7% ）. SiHMA showed tissue-specific responses toCdandFestress.Theposible functionsof SiHMA were predicted.Functionalpredictionssuggested thatSiHMA5mediated Zn ，Cu，Pb and Cd eflux，while SiHMA1O might be involved intheloading and transportation of Cu in plants.[Conclusion]TandemandsegmentalduplicationdroveHMAfamilyexpansionand functionaldiversificationin foxtail millet.Cis-actingelementsrelatedtoplanthormonesandstressresponseswereidentifiedinSiMApromoters.Basedonho mology，expresson paterns，and previous evidence，we proposed roles for SiHMA1，SiHMA5，SiHMA6and SiHMA10 in metal homeostasis.These results provide a theoretical basis for further studyon HMA genes in foxtail milet.

Keywords：Foxtail milet，HMA genefamily，Homologousanalysis，Expression profiling，Functionalprediction

Cu、Zn和Fe等许多金属元素是植物生长发育的必需金属元素，广泛参与各种生化和生理过程，它们作为酶的辅助因子，在细胞氧化还原反应中发挥作用，也会影响蛋白质的空间结构、稳定性和信号转导[1]。（剩余21485字）