L-茶氨酸通过Nrf2/HO-1通路减轻H9c2心肌细胞缺氧再复氧的氧化应激和细胞凋亡

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【Abstract】ObjectiveToinvestigatetheefectsofL-theanineonhypoxia-reoxygenation（HR）-inducedinjuryinH9c2 cardiomyocytesanditsuderlyingmechanisms.MethodsAnHRinjuryodelasestablishdinH9c2cardiomyoces.Cellreadomly divided into the following groups：normoxia group，HR group，and HR+L-theanine （2，4，8 mmol/L） groups，and HR + L-theanine（8 mmol/L） + （20 ML385group.Cellviabilityasmeasuredusingcellcountingkit-8assLevelsofreactiveoxygenspeies，alondialdehdeupoide dismutase，cataaecatedrogastiessddacrinsedicali wasdetectedbteialdeoxyucleotidyltrasferas-mdiatedUickndabelinsainngProteinexprsioofcl-asiatedX protein，B-cellp2asps-learactordrelatedfctorndyena-aseedyWstloi MeengerRNAexpresionofuclearfactorerythroid-relatedfactor2andhemeoxygenase-1wasdetectedbyquantitativeeal-tie polymerasechainreactio.ResultsHRstimulationsignficantlyducedcellviabiltyndicreasedtelevelsfocaialijuryarks lactate dehydrogenase，creatine kinase isoenzyme，and cardiac troponin I（ P<0. 05 ）.Pretreatment with L-theanine effectively restored cell viability and reduced the release of injury markers （ P<0 . 05）.HR-induced oxidative stress was characterized by elevated reactive oxygen speciesandmalonddehdelevelsanddecreasedsuperoxidedismutaseandatalaseactivityhichweresignificantlyeversdbyhnine intervention（ P<0 .05）.Additionally，HR groupshowed significantly increasedapoptosisrateand caspase-3protein expresion，with upregulated-ooraelloaptt （ P<0. 05 ）.Furtherstudyfoundthatnuclearfactorerythroid2-relatedfactor2andhemeoxygenase-lexpressonwassignificantly downregulatedinHgoup，whileLeanineintervetionestoredteirexpresiontolevelslosetoomoxiagroup.Thenuclearfactor erythroid 2-related factor2 inhibitor ML385 reversed the above protective effects of L-theanine（ P<0.05 ）.Conclusion L-theanine attenuates oxidativestresandapoptosisinHc2cardiomycytesxposedtohypoxia-reoxygeationijuryyactivatingteuclearfactoroidrelated factor 2/heme oxygenase-1 pathway.

【Keywords】L-theanine;Cardiac muscle cell; Hypoxia-reoxygenation;Oxidative stress;Apoptosis

急性心肌梗死（acutemyocardialinfarction，AMI）作为全球范围内致死率最高的心血管事件之一，其病理过程与冠状动脉急性闭塞导致的持续性缺血密切相关，一直被社会关注[]。（剩余12223字）