耐高温秸秆降解菌筛选鉴定及降解特性研究

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中图分类号：X172；X71 文献标志码：A 文章编号：1672-2043（2025）07-1910-10

doi：10.11654/jaes.2024-0790

Screening，identification and degradation characterization of high-temperature-tolerant straw-degrading bacteria

YINLiwen’，MO Yingping1，GENG Tianzhi1，ZHANGNa'，LIU Cuiying2，FANJianling1* （1.JiangsuKeyLboatoofmosicEvioentotongndlltioontrol，iagsuPrincialColborativion CenterofAtmosicEiontdEqptTlghoolofEiotalicedEginering，angUi Inforatioiedcholin，a;.Jgybatorfaleeoof Applied Meteorology，Nanjing Universityof InformationScience and Technology，Nanjing ，China） Abstract：Teectiveoftisstudyastoentifygempeatureligocellosicegdingcteriafroapdysoilablisha consortiumofteigeinoellosicaaodeatesadaiotoee thecomprehensiveutilizationofagriculturalwaste.Weobtained11degradingstransthroughpreliminaryscreningbyincubatinfor enrichmentcultureandusingCongedstainingandanilinbluefadingreaction.Theenzymaticatvitiesofcelulose，hemicelloseand lignin were measured.The highest β -Dglucosidase activity was found in K6（716U⋅mL-1， ，thehighest cellulase activitywas obtained in K1（393 U·mL-1），and the highest hemicellulase activity was found in K4（439U⋅mL-1 ）. The highest lignin peroxidase activity was found in strain R3（6.213 U·mL-1），the highest manganese peroxidase activity found in R1（3.989U⋅mL-1） ），while the highest laccase activity was found in strain K5（2.593U⋅mL-1） .Based on the results of the transparent circle test， enzymatic activities of cellulose，hemicelllose and lignin，sixstras（2，，R4K25ndK6）reeeedforoleulardetfatisusodatsolae toPriestiamegtesoyadtcilsubils4aselyeatedtllucfsasely Pristiaarybatai，K5andK6wereloselelatedtBcilluselezesisTreecosoaeeconstructedbyusingsinglesrasWe foundthatonsortiumC（R4andK6）hadthestdegradingefect，withthefilerpaperstripegiingtodisintegrateodaanding intopaperflossbyday5.After15days，testrawdegradationrateas2.69%ighertanthatoftecontrol.Theresultssugesthathe degradation effect of consortia was better than that of singlebacterial colony.

Keywords：thermotolerant;strawdegradingbacteria;identification;degradationcharacteristic;compositebacterial

据统计，目前我国农作物秸秆年产量超8亿t，且总体产量呈缓慢上升趋势[]。（剩余22185字）