川陈皮素调节TRPV1信号通路对OGD/R诱导的神经细胞凋亡的影响
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AbstractObjective：ToexploretheefectNobletinOB）oneuronalapoptosisinducedbyoxgenglucosedeprivation/reoygetio （OGD/R）byregulatingterasientreceptorpotentialvanilidsufamil1PV）signalingpathayMethods：PC12cellseeuure inprimarycultureandanGD/RijuymodelwsestablishedAftertreatigGD/iducedPC12cllswit0，1030，4，d6 μmol/mL NOB.Thiazolyl blue（MTT）assay wasapliedtodetectcellsurvivalrates ineach groupandscreen fortheoptimal concentrationNOB.PC12celsculturedinvirowererandomlyseparatedintoacontrolgroup（untreated），OGD/Rgroup（GD/R intervention in PC12 cells），OGD/R + NOB group（OGD/R+50 μmol/mL NOB co intervention in PC12 cels），and OGD/R+NOB+RTX （TRPV1activatorgroup（OGD/R+50μmo/LNOB10nmoRTXcointerventioninPC12cell）Tassaandflowcyometrywere apliedtodetectthesuvivalrateandapotosisatecelinachgropReagentkitmethodasapliedtodetectelevelactate dehydrogenase（LDH），alondialdehde（MDA）uperoxidedsmutase（SOD），educedglutathione（GSH），andmornecrosisfacto α（TNF-α） inPC12celsWstaldtoeectthexesso1，Blobsto-2c-2）ndc-2soaedotein （Bax）proteinsutsCoadietrooptalateOvesdc-2otenepreionin in the OGD/R group were reduced P<0.05 .Compared with the OGD/R group，the the survival rate，SOD，GSH levels，and Bcl-2 protein expression in the OGD/R+NOB group increased .Compared with the OGD/R + NOB group，the survival rate，SOD，GSH levels， and Bcl-2 protein expression in the OGD/R+NOB+RTX group decreased（ P<0.05） .Comparedwith the control group，the apoptosis rate，LDH，MDA，TNF- α levels，TRPV1，and Bax protein expression inPC12 cels in the OGD/R group increased ⌈P<0.05⌉ .Comparedwith the OGD/R group，the the apoptosis rate，LDH，MDA，TNF- α levels，TRPV1，and Bax protein expression inPC12 cels in the OGD/R + NOB group decreased（ P<0.05 .Compared with the OGD/R + NOB group，the apoptosis rate，LDH，MDA，TNF- ∝ levels，TRPV1，and Bax protein expression inPC12 cells in the OGD/R + NOB + RTX group increased（ P<0.05 ）.Conclusion：NOB can reduce OGD/R induced inflammatoryesponseandoxidativestresslevelsbyinhibitingtheactivationTRPV1signalingpathwayutimatelyaleviatingGD/R induced PC12 cell apoptosis.
Keywordsygnucoedepriatioogeatio;etin;astetorpotetialnilsubmil;urastosis
缺血性脑卒中（ischemicstroke，IS）是全球人类死亡和残疾的主要原因之一，通常由血栓突然阻塞脑血管，使脑部血、氧供应不足进而引起的神经性系统疾病[1]。（剩余9494字）
