瑞芬太尼调节PI3K/AKT/mTOR信号通路对oX-LDL诱导的血管平滑肌细胞增殖、凋亡及迁移的影响

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doi：10.12102/j.issn.1672-1349.2026.01.009

AbstractObjective：Toexplorethefectofremifentanilonthephosphatidylinositol3kinase（Pl3KproteinkinaseB（AKT/ammlan targetofrapamycinOR）signalingpathwayinegulatingtheproliferationpoptosisandmigrationofascularsmoothlecels （VSMCs）inducedidsityliote-o：didintotroouputreat group 50mg/L ox-LDL），L-remifentanil group （0.5ng/mL remifentanil），M-remifentanil group（ 5.0ng/mL remifentanil），H-remifentanil group remifentanil），and740Y-Pgroup remifentanil+10 μmol/L Pl3K activator 740Y-P）.The Cell Counting Kit 8（CCK-8） kitwasusedtodetecttheprolferationofVSMCs.TheflowcytometerwasusedtodetecttheapoptosisofVSMCs.Thescratchassaywas usedtodetectthemigrationof VSMCs.TheTranswellmethodwasusedtodetectheinvasionof VSMCs.Theproteinimmunobloting method（WesternBlot）wasusedtodetect heproteinexpression levelsofPl3K，phosphorylatedphosphatidylinositol3-kinase 1 Πp -Pl3K）KTootedeiedspotedagtfi： Compared with thecontrol group，theODvalue，scratch healing rate，invasion number，p-Pl3K/Pl3K，p-AKT/AKT，and p -mTOR/mTOR proteinexpressions in the ox-LDL group increased，while theapoptosis rate decreased（ P<0.05） .Compared with the ox-LDL group，the ODvalues，scratch healing rate，invasionnumber，protein expressionsof p -PI3K/PI3K，p-AKT/AKT，and p -mTOR/mTOR in the L-refrinixin group，M-refrinixin group，and H-refrinixin group decreased，while the apoptosis rate increased P<0.05 .Comparedwith the H-refrinaxin group，the OD value，scratch healing rate，invasion number，expression of p -PI3K/PI3K，p-AKT/AKT，and p -mTOR/mTOR proteins in the 74oY-P group increased，while the apoptosis rate decreased（ P<0.05） . Conclusion： Remifentanil can further inhibit the proiferationandmigrationofsmoothmusclecellsandpromotetheirapoptosisbysuppressngtePl3K/AKT/TORsignalingpathway

Keywordsvascularsmoothmusclecell;remifentanil;phosphatidyinositol3kinase/proteinkinaseBmammaliantargetofrapamycin signaling pathway;oxidized low-density lipoprotein;proliferation;apoptosis;migration

动脉粥样硬化（atherosclerosis，AS）是一种由于脂代谢发生紊乱而导致的血管疾病，常常会引起冠心病、心肌梗死等一系列的并发症，对人们的生命健康造成严重威胁[1]。（剩余9499字）