疫霉菌株XC-26-5的分离及其生防制剂对烟草黑胫病的防效

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中图分类号：S435.72 文献标识码：A文章编号：1007-5119（2026）01-0097-09

Abstract：Toachieve thegreenpreventionandcontrolof tobacco blackshank，Phytophthoranicotianae wasisolatedusingtisue analysis combied withmolecularidentification.Through pathogenicitytestsandoomycete virus detection，ahypovirulentstrain XC26-5carryinga viruswasscreened.Folowing shake-flask cultivationthemicrobial agent wasprepared via emulsificationand shearing.Fieldexperimentswereconducedtoinvestigatetetredsaddifferenesintheefectsofdierentaplcationatsofth biocontrol agent on the abundance of P . nicotianae in soil， incidence rate， disease index，and control efficacy. Amplicon sequencing was employed toanalyze itsimpactonthe diversityand communitystructureof tobacco rhizosphere soil microrganisms.Results showed thatthehypovirulentstrain XC-26-5grewslowlyonPDAmedium，withshortersporangiophoresandreducedsporangia，nd carried the partitivirus PnPV1 （Phytophthora nicotiana partitivirus 1）. Field trials indicated that when the XC-26-5 agent was applied at 4.50kg/ha ，the soil pathogen population decreased by 50.1% ，the incidence ofblack shank was reduced by 69.73% ，and the control efficacy reached 70.1% .High-throughput sequencing revealed that XC-26-5 significantly increased the Simpson， Chaol，and ACE indicesofsoilcobaldivesitylteredmicoialommunitytructureandichdbeneicialgerauchasngomoaand Lysobacter in the rhizosphere.The virus PnPV1caried by XC-26-5 effectively reduced the pathogenicityand sporulationof P nicotianae，achieving robust control of tobacco black shank disease. Application of XC-26-5 at 4.50kg/ha demonstrated optimal efficacy in pathogen suppression and soil microbiome modulation.

Keywords： hypovirulent Phytophthora; control of tobacco black shank; mycovirus; soil microbiome

烟草黑胫病由烟草疫霉Phytophthoranicotianae引起，其发生与根际土壤中烟草疫霉病原菌的数量密切相关，病原菌可在土壤和残茬中生存，因此防治较为困难。（剩余15155字）