贝莱斯芽孢杆菌DY-6-6的筛选及对烟草黑胫病的生防潜力

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中图分类号：S435.72 文献标识码：A文章编号：1007-5119（2026）01-0088-09

Abstract：Tobaccoblack shank，causedbyPhytophthora parasiticavar.nicotianae infection，isa major soil-borne diseaseof tobacco.Toachievethesustaiableandeffectivemanagementofthisdisease，thisstudyscreenedbiocontrolstrainsfromderent tobacco-growingregions，valuatedteirstress tolerance，entifiedteirtaxonomicstatus，determinedteiratifungalmchnism againstthtargetpathogenandassedteirdiseasesuppressieadplantgowthpromotingactivities.esultsevealedat totaloffourbiocontrostrans withhighantifungalactivitywereisolatedandscreened.Amongthesestrains，D-6-6exhibitedthe optimal antifungal activity and stress tolerance，and was identified as Bacillus velezensis. Treatment with B .velezensis DY-6-6 led to anincrease imalondialdehyde（MDA）content，adecreaseinsuperoxidedismutase（SOD）activitycatalase（CAT）activitynd protein content of P parasitica，as well as ahigh biofilm-formingcapacity. B .velezensis DY-6-6 exerted both germination-promoting andgrowth-promotingefectsontobacco，astheseed germinationrate，leafarea，plantheight，andbiomassweresignificantly increased after treatment. The relative preventive effect of B .velezensis DY-6-6 fermentation broth on tobacco black shank in pot experiments under controlled indoor conditions was 70.35% ，andthe relative therapeutic effectwas 59.20% ，which wasconsistent withthatof thefungicide.Theseresultsprovideanewbiocontrol train forthe managementof tobacco blackshankandclarifyits disease-suppressive and growth-promoting mechanisms.

Keywords： tobacco black shank; plant growth-promoting rhizobacteria; Bacilus velezensis; biological control

烟草是我国重要的经济作物之一，种植面积和总产量均居世界首位[1]。（剩余15262字）