不同生物刺激素对烤烟碳氮代谢的影响

打开文本图片集

中图分类号：S572；TQ450.1 文献标识码：A文章编号：1007-5119（2026）01-0031-08

Abstract：Inorder toexploretheeffectsof different biostimulantsoncarbonand nitrogenmetabolismofflue-cured tobacco， Zhongyan 1Oo was used as the material， and four treatments of spraying water （CK）， 100mg/L γ -aminobutyric acid （T1）， 100mg/L chitosan oligosaccharide（T2）and 100mg/L alginate oligosaccharide（T3） were set up. The biomass， photosynthetic characteristics andcarbonand nitrogen metabolismoflue-cured tobaco were analyzedbyfoliarsprayingat the vigorous growthstageand mature stageof tobaccoplants.Theresultsshowedthat，（l）Comparedwiththecontroltreatment，biostimulanttreatmentimprovdthe photosynthetic characteristics of tobacco leaves， the net photosynthetic rate increased by 13.2%-33.3% ， respectively， and the biomass accumulationofflue-cured tobacco increased.Inthevigorous periodofcarbonand nitrogen metabolismofflue-curedtobaccothe activities of nitrate reductase， glutamine synthetase， neutral invertase and αa -amylase in tobacco leaves were significantly increased， andtheaccumulationand transformationof carbonandnitrogen metabolitesin tobaco leaves were promoted.T1treatment significantlyincreasedtheactivityofcarbon metabolismenzymes，promoted theacumulationof totalsugarandreducing sugarin tobaccoleaves，and theexpression levelsofcarbon metabolism genes NtNINV10，NtAMMLand NtTPPwere significantlyupregulated.T2treatmentsignificantlyincreasedthactivityofnitrogenmetabolismenzymesinflue-curedtobaccopromoteditrogen absorptionandicotiesthesisinflecuredtobaccoandtheexpressionlevelsoftrogenmetabolismgenesNN，d NtGDH1were significantly increased.T3treatment increased theactivity and productsof carbon and nitrogen metabolismenzymes influe-curedtobacondsigniicantlyincreasedtheexpressionlevelsof NAM.Insummary，spraingdierentbiosiulants significantly promoted the growth and development of tobacco plants. Among them， γ -aminobutyricacid significantlyaffected the carbon metabolismprocessoffue-cured tobacco，chitosanoligosaccharidesignificantly increased thentrogenmetablismntesity offlue-credtobaccoandalginateoligosaccharidehadabalancedeffectonthecarbonandnitrogenmetabolismofflue-cured tobacco.

Keywords： flue-cured tobacco; biostimulant; carbon and nitrogen metabolism; gene expression

生物刺激素作为一类生物活性制剂，能调节植物生理过程，增强植物营养吸收，激活植物信号传导途径且与环境具有良好的相容性，能有效提升烟叶品质，被认为是实现农业绿色可持续发展的重要措施[1-2]。（剩余13189字）