黄花苜蓿MfWRKY3基因克隆及特性分析

打开文本图片集

中图分类号：S541.9 文献标识码：A 文章编号：1007-0435（2026）03-0796-08

doi：10.11733/j.issn.1007-0435.2026.03.006

引用格式：，等.黄花苜蓿MfWRKY3基因克隆及特性分析[J].草地学报，2026，34（3）：796－803 ZHAO Jing-wei， JIN Xiao-wei，CUI Meng-lan，et al. Cloning and Characterizatic Analysis of MfWRKY3 Gene in Medicago falcata[J].Acta Agrestia Sinica，2026，34（3） ：796-803

Abstract：To explore the role of MfWRKY3 in regulating the response of Medicago falcata to stresses，the MfWRKY3 gene was cloned from wild Medicago falcata in this study，and the characteristics of it encoded pro tein and the expression patern were preliminarily identified.The results showed that the length of MfWRKY3 ORF（open reading frame） is 1575 bp，encoding a protein with 524 amino acids，predicted molecular mass of 57.36kDa and an isoelectric point of 6.96. The MfWRKY3 had transcriptional activation activity and was localized in the nucleus，belonging to the WRKY I sub-group transcription factor member. Meanwhile，it was found that the MfWRKY3 gene had the highest expression level in the leaves of Medicago falcate，and the its expression in the roots， stems and leaves were regulated by salt，low temperature and ABA treatment. However，the salt stress could significantly induce the expresson of MfWRKY3 gene. These findings laid a foundation for further investigating the biological functionand molecular mechanism of MfWRKY3 in regulating the response of Medicago falcata to abiotic stresses.

Key words：Medicago falcata;MfWRKY3;Gene cloning;Characterization analysis

WRKY转录因子家族蛋白在植物的生长发育和响应各种胁迫中发挥着重要的调控作用[1-2]，其通过WRKY结构域与靶基因启动子区顺式作用元件W-box特异性结合，调节靶基因的表达[2]。（剩余13492字）