发酵黏液乳杆菌FUA033 高密度发酵培养基及发酵条件优化

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中图分类号：TS205.5 文献标志码：A 文章编号：1000-9973（2026）01-0028-08

DOI：10.3969/j.issn.1000-9973.2026.01.004

Dptimization of High-Density Fermentation Medium and Fermentation Conditionsof Limosilactobacillus fermentum FUA033

TANG Ming1，SU Ya-jian1，WU Qing1，FANG Yao-wei1，ZHANG Wen-jun1 ， LIU Shul＊，HOU Xiao-yue¹，CHEN Tao² （1.School of Ocean Food and Biological Engineering， Jiangsu Ocean University，Lianyungang 222005，China；2.Suqian Product Quality Supervision and Testing Institute， Suqian 223800，China）

Abstract： Limosilactobacillus fermentum FUAo33 converts ellagic acid into urolithin A with multiple biological activities，which is expected to be developed into a new generation of probiotics. This study aims to optimize high-density fermentation medium and fermentation conditions of the strain to reduce the preparation cost of the strain.The medium and fermentation conditions of Limosilactobacillus fermentum FUA033 are optimized by single factor experiment and response surface experiment. The results show that the optimal fermentation medium of Limosilactobacillus fermentum FUA033 is 3.1% glucose， 1.6% peptone， 0.5% （20 K2HPO4 ， 0.5% yeast powder， 0.5% beef extract powder， 0.1% Tween 80， （204号 0.5% sodium acetate， 0.2% ammonium citrate， 0.005% manganese sulfate and 0.02% magnesium sulfate. The optimal fermentation conditions are pH of 6.8，temperature of 38°C ，inoculation amount of 2.9% and fermentation time of 20h . The viable bacterial count in the fermentation broth reaches 2.06×1010 CFU/mL，which is 8.77 times that before optimization. The results of this study have laid an experimental foundation for the improvement of viable bacterial count of Limosilactobacillus fermentum FUAo33 direct vat set bacterial agent and the further development and application.

Key words： Limosilactobacillus fermentum； single factor experiment； response surface experiment; medium optimization；optimization of fermentation conditions

鞣花酸是一种广泛存在于多种果蔬中的多酚[1]。（剩余10704字）