连续继代培养对羽枝青藓生长及内源激素的影响

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中图分类号：S731.6 文献标志码：A 文章编号：1673-923X（2026）02-0100-11

Abstract：【Objective】Thecultivationandrestorationofockmoscrustsispotentialnewmeasureforecologicalrestorationinthe Qinlingminingea.Howeer，teisufentsuplyofosedsouesasbcoeasignicantbotteneckinpraticalacation Exploringthegeneratioalangsinteowthdogenousoeiicatosndlozationabiltyfacthiuosum throughsuccesivesubculturesusing imitednaturalmossscanprovidetheoreticalsupportfortelargscalesubcultureandepasio ofseedsurces，whichisofgeatsignificancefortheindustrialzationofmoscrustecologicalrestoratio.【Method】Thedoant speciesBrachytheciumplumosumfromrock-dwellngbiocrustswassubjectedtothreesuccessivesubcultureccles（30daysper generation）underontrolldlaboratoryonition.tergenrationaldirenesinoveragenewbranchlngthranchumbe andsix endogenoushormones（includingIAAandABA）wereanalyzed，withtheircorrelationsexamined.【Result】1）Suesive subculturesinducedsigfcantarationsinedogeoushomoepoflesacrossgeneratios，otentiallyexplainingobseedgrowth indexdisparities;2）Endogenous hormones exhibitedvaryingcorelations withgrowthindices：IAAshowedpositivecorrelations withgrowthparameters，whereasABAdisplaednegativeoelations，withteserelationsipsdependingondevelopmentalstages; 3）Afterthrconsecutivesubultures，theindoo-cultivatedBrachythciumplumosumstillmaintaedahighpropagationeciency After32daysofoutdoorcolonzation，therewerenosignfcantdiferencesingrowthidicatorsamongdiferentsubculturegenetios. 【Conclusion】The endogenous hormone contents of indoor-cultivated Brachythecium plumosum showedobviousgenerational diferences，andtecorelationswithgowthindicatorsvariedwiththegowthanddevelopmentstagesofBrachyteciumplumosum. Thesefactorsmightjointlycausethegenerationaldfferencesingrowthindicators.ThegowthindicatorsofBrachytheciumplumosum couldstillmaitainaighleveattendofultivationaftersucessiesubulture，ndterewrenogeneratioaldieecesin outdoorcolonzationabilitydicatingthatsubultureexpasioncanbeusedtoontinuouslybtainBrachytheciumplumoud sources.

Keywords：Brachytheciumplumosum;subculture;growth;endogenoushormones;intergenerationaldifferences

秦岭是中国重要的资源宝库，在地理、气候和生物多样性等方面有着重要意义[。（剩余19801字）