基于ISSR标记的野茶资源遗传多样性分析

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中图分类号：S571.1 文献标识码：A 文章编号：2095-5774（2025）06-0640-06

Genetic diversity analysis of wild tea plant resources based on ISSR markers

Feng Hongxuan

（Economic Crop Technology Promotion Center of Shaxian District，Sanming，Fujian 365O50， China

Abstract：【Objective】 Shaxian County has abundant resourcesof wildtea plant Camelia sinensis，and conducting phylogeneticanalysisoftearesourcesinthearea hasgreat significance for itsresearch，protection，andutilization. 【Method】Basedon ISSR molecular marker technology，the genetic relationship of 16 wild teaand 5 cultivated resources in Shaxian Countywasanalyzed.NTSYSsoftware wereused for genetic similarityclusteringanalysisand principal coordinate analysis.Result】A totalof9ISSR primers were screened，and88amplifiedbands were detected，including74 polymorphic sites，withapolymorphismrateof84.O9%.Clusteranalysisand principal coordinateanalysis revealed that whenthegeneticcorrelationcoeficientreachesO.68，21tearesourcescanbedivided intothree categories.Rougui'has thefarthest genetic distance fromother species，wild tearesources such as HuyuanandNanyang haveaclose genetic distance from‘Shuixian'，'Huangdan'，'Maoxie'，and 'Tieguanyin'，whileresources inDaluo Townand Dayoushan havea far genetic distance from the reference cultivated species.【Conclusion】 Shaxian County hasabundant wild tea resources， complex genetic diversity，and strong potential for exploring excellent germplasm.

Keywords：wild teaplant;ISSR; primer;cluster analysis; Principalcoordinate analysis （PCoA）;genetic distance

茶（Camelliasinensis）隶属于杜鹃花目（Eri-cales）山茶科（Theaceae）山茶属（Camellia），主要分布在东亚及东南亚地区。（剩余5427字）